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Bovine Alpha-2-macroglobulin(A2M) ELISA kit

Citations (1) Protocol
Code CSB-EL001004BO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Bovine A2M ELISA Kit was designed for the quantitative measurement of Bovine A2M protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.312 μg/mL- 20 μg/mL and the sensitivity is 0.078 μg/mL.
Target Name alpha-2-macroglobulin
Alternative Names A2MAlpha-2-macroglobulin ELISA kit; Alpha-2-M ELISA kit
Abbreviation A2M
Uniprot No. Q7SIH1
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 μg/mL- 20 μg/mL
Sensitivity 0.078 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine A2M in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 98  
Range % 90-103  
1:200 Average % 90  
Range % 82-94  
1:400 Average % 101  
Range % 95-106  
1:800 Average % 108  
Range % 101-112  
Recovery
The recovery of bovine A2M spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 88 82-91  
EDTA plasma (n=4) 93 87-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
20 0.127 0.131 0.129    
10 0.248 0.233 0.241    
5 0.490 0.523 0.507    
2.5 0.909 0.911 0.910    
1.25 1.439 1.464 1.452    
0.625 1.853 1.789 1.821    
0.312 2.460 2.400 2.430    
0 2.795 2.702 2.749    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Gene References into Functions
  1. Mutations in the promoter region and the 3'-untranslated region of the A2M gene are involved in the mastitis susceptibility in dairy cows. PMID: 25237709
  2. We report here for the first time the direct isolation of Spp24 covalently bound to alpha2-macroglobulins as a core complex (Spp24-alpha2M) from bovine calf serum. PMID: 22949401
  3. Exon 29 c.3535A>T in the alpha-2-macroglobulin gene causing aberrant splice variants is associated with mastitis. PMID: 22923050
  4. These results suggest the possible involvement of cathepsin E in disruption of the structural and functional integrity of alpha 2-macroglobulin in the endolysosome system. PMID: 12631277
  5. Alpha2-macroglobulin is a substrate and an endogenous inhibitor for ADAMTS-4 and ADAMTS-5 PMID: 14715656
  6. Amounts of alpha(2)-M receptor in granulosa cells were strongly inversely associated with concentrations of estradiol in dominant and subordinate follicles. PMID: 15001551

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Subcellular Location Secreted.
Protein Families Protease inhibitor I39 (alpha-2-macroglobulin) family
Tissue Specificity Plasma.
Database Links

KEGG: bta:513856

STRING: 9913.ENSBTAP00000006167

UniGene: Bt.60794
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