Bovine Interleukin-18(IL18) ELISA kit

Code CSB-EL011608BO
Size 96T,5×96T,10×96T
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Product Details

Target Name
interleukin 18 (interferon-gamma-inducing factor)
Alternative Names
IL18 ELISA Kit; IGIF ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
Abbreviation
IL18
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.81 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 88
Range % 83-92
1:2 Average % 97
Range % 91-105
1:4 Average % 95
Range % 92-99
1:8 Average % 93
Range % 87-98
Recovery
The recovery of bovine IL18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 90-110
EDTA plasma (n=4) 97 90-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 3.011 2.978 2.995 2.900
1000 2.511 2.522 2.517 2.422
500 1.496 1.504 1.500 1.405
250 0.705 0.716 0.711 0.616
125 0.382 0.375 0.379 0.284
62.5 0.226 0.234 0.230 0.135
31.25 0.152 0.159 0.156 0.061
0 0.093 0.097 0.095  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Bovine IL18 ELISA Kit was designed for the quantitative measurement of Bovine IL18 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

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Target Background

Function
(From Uniprot)
A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells.
Gene References into Functions
  1. These results suggest that BAPC-1 is a stem/progenitor cell line and modulates the immuno-endocrine function of the anterior pituitary cells through its production of interleukin-18 and the IL-18 receptor. PMID: 18761716
Subcellular Location
Cytoplasm. Secreted.
Protein Families
IL-1 family
Database Links

KEGG: bta:281249

STRING: 9913.ENSBTAP00000055793

UniGene: Bt.234

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