Bovine Leptin,LEP ELISA Kit

Instructions
Code CSB-E06771b
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name leptin
Alternative Names LEP ELISA Kit; OB ELISA Kit; OBS ELISA Kit; Leptin ELISA Kit; Obesity factor ELISA Kit
Abbreviation LEP
Uniprot No. P50595
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 ng/mL-50 ng/mL
Sensitivity 3.12 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 89-98  
1:2 Average % 90  
Range % 87-94  
1:4 Average % 89  
Range % 85-94  
1:8 Average % 100  
Range % 96-105  
Recovery
The recovery of bovine LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 90-96  
EDTA plasma (n=4) 90 85-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
50 0.262 0.253 0.258    
25 0.520 0.512 0.516    
12.5 0.831 0.877 0.854    
6.25 1.716 1.668 1.692    
3.12 2.439 2.519 2.479    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)
Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways (By similarity). In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity (By similarity). In the arcuate nucleus of the hypothalamus, activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides (By similarity). In addition to its known satiety inducing effect, has a modulatory role in nutrient absorption. In the intestine, reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38, PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption (By similarity). Acts as a growth factor on certain tissues, through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1, via JAK2-STAT3 pathway, or VEGFA, via MAPK1/3 and PI3K-AKT1 pathways (By similarity). May also play an apoptotic role via JAK2-STAT3 pathway and up-regulation of BIRC5 expression. Pro-angiogenic, has mitogenic activity on vascular endothelial cells and plays a role in matrix remodeling by regulating the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). In innate immunity, modulates the activity and function of neutrophils by increasing chemotaxis and the secretion of oxygen radicals. Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators. Increases cytotoxic ability of NK cells. Plays a pro-inflammatory role, in synergy with IL1B, by inducing NOS2 wich promotes the production of IL6, IL8 and Prostaglandin E2, through a signaling pathway that involves JAK2, PI3K, MAP2K1/MEK1 and MAPK14/p38 (By similarity). In adaptive immunity, promotes the switch of memory T-cells towards T helper-1 cell immune responses (By similarity). Increases CD4(+)CD25(-) T-cell proliferation and reduces autophagy during TCR (T-cell receptor) stimulation, through MTOR signaling pathway activation and BCL2 up-regulation (By similarity).
Gene References into Functions
  1. Consistent with this, leptin enhanced GnRH-induced secretion of LH measured by ELISA. We suggest that leptin enhances membrane expression of voltage-gated Na(+) and Ca(2+) channels, which results in a modulation of the action potential properties and an increase in hormone release from gonadotropes. PMID: 28705737
  2. The effects of a single nucleotide polymorphism of leptin on weight gain and body composition are reported. PMID: 28177355
  3. A leptin SNP (LEPg.978) was significantly associated with a weight at one year. PMID: 27706683
  4. The results of this study suggest that leptin physiology could be a candidate for mechanisms that contribute to feed intake and feed efficiency variation in beef cattle. PMID: 26851619
  5. Leptin concentrations could be a useful physiological marker for growth and feed efficiency of finishing beef cattle. PMID: 26440340
  6. Hormonal gene expression involved in residual feed intake in dairy cows may be related to the molecular regulation of the leptin-NPY and insulin signaling pathways. PMID: 26231801
  7. Leptin R25C genotype impacted most traits associated with fatness. PMID: 24753381
  8. The absence of A80V polymorphism (C --> T at position 95691973 bp of leptin gene) has been established in the genotypes of Ayrshire cattle as compared to Holstein cattle. PMID: 25966594
  9. Data indicate that increased body weight gain during juvenile development accelerates sexual maturation in heifers, coincident with reciprocal changes in circulating concentrations of leptin and hypothalamic neuropeptide Y (NPY) release. PMID: 25326602
  10. It is an important regulator of metabolic efficiency, energy expenditure, food intake and body fat mass. PMID: 23910951
  11. SNP LEP significantly affected milk, protein and fat yield (P<0.05), and age at first calving (P<0.01) in analyzed population of cows. SNP LEPR/T945M affected significantly calving interval (P<0.01) only PMID: 24432332
  12. Results indicated that leptin R25C genotype impacted most traits associated with fatness whereas feeding zilpaterol for 21 d affected weight gain positively but impacted other variables negatively. PMID: 23942708
  13. There is a strong association between putative favorable allelic variants (SNP) of neuropeptide Y, leptin, and IGF-1 genes, and residual feed intake, when animals were grazing on a high-quality, high-availability pasture. PMID: 23881687
  14. The LEP, IGF2 and CCL2 genes showed allelic expression imbalance in liver, kidney and pituitary glands of Polish Holstein-Friesian bulls. PMID: 23184004
  15. The aim of the study was to determine by immunochemistry the expression of leptin, orexin A and orphanin FQ in the major salivary glands (parotid, submandibular and sublingual) of rat, sheep and cow. PMID: 23264212
  16. The use of leptin, leptin receptor, and DGAT1 polymorphisms as markers within genetic selection programs to improve and adjust several compositional parameters. PMID: 22127264
  17. The leptin and receptor gene markers would be useful for marker-assisted selection. PMID: 22930433
  18. A significant positive relationship exists between the T allele frequency in the bovine leptin C/T SNP and animal backfat, with TT, CT and CC animals having estimates of 6.79 +/- 0.02, 6.49 +/- 0.01 and 6.28 +/- 0.01 mm, respectively. PMID: 22497203
  19. The impact of different alleles of DGAT1 and leptin on milk production in Giorlando cattle is reported. PMID: 21396671
  20. The effects of dietary energy on the metabolism of ghrelin, leptin and growth hormone secretagogue receptor in blood and tissues of steers are reported. PMID: 21724941
  21. This study provides evidence that leptin gene polymorphisms play a role in the variability of hematological variables during the peripartum period, and might be used as genetic markers to improve dairy cow immunological conditions in productive periods. PMID: 21890501
  22. This study reports changes in plasma leptin concentration parallel to changes in the gene expression of lipogenic- and lipolytic-related genes in adipose tissue of dairy cows around parturition. PMID: 21198961
  23. Gene expression of leptin was affected by parental origin in cattle. PMID: 21899958
  24. Cows homozygous for the T allele were less likely to be cyclic early postpartum, but leptin genotype was not associated with other reproductive responses when cows were subjected to a controlled breeding program. PMID: 21917388
  25. Mixed model analyses revealed that leptin SNP were associated with early skeletal growth (height, A1457G; length, A59V), fertility (UASMS1, UASMS2, A1457G, A59V) and milk production (A59V). PMID: 21700051
  26. Fundamental role of SCD1 genes in transforming saturated fatty acids into monounsaturated fatty acids is mediated by the leptin gene. PMID: 21145173
  27. polymorphisms in the leptin gene were associated with 2-fold differences in perinatal mortality in dairy heifers PMID: 20059932
  28. the possible involvement of locally produced leptin/Ob-R system in the bovine ovary, suggesting roles in the function and/or development of the CL and growth of small follicles in an autocrine/paracrine fashion. PMID: 19908249
  29. Leptin tended to suppress serum growth hormone in cattle PMID: 19632078
  30. Genomic structure and promoter analysis of the bovine leptin gene PMID: 12049196
  31. higher leptin concentrations associated with shorter intervals to first observed estrus might indicate a relationship between leptin and expression of estrus PMID: 12703616
  32. insulin increases plasma leptin in late pregnancy by stimulating adipose tissue synthesis but has only marginal effects in early lactation, when cows are in negative energy balance PMID: 12881203
  33. findings indicate that acetate and butyrate enhance leptin expression in bovine, but not in rat anterior pituitary cells while butyrate suppresses leptin receptor expression in both rat and bovine pituitaries PMID: 12928006
  34. exogenous leptin can prevent fasting-mediated reductions in the frequency of LH pulses and modify GnRH-mediated release of LH in intact, prepubertal heifers. PMID: 14522828
  35. cattle homozygous for the leptin T allele produced more milk and had higher somatic cell count PMID: 14594235
  36. plasma leptin is regulated by nutrition in early postnatal life, but that a sudden increase in plasma leptin is not required for the onset of puberty in dairy cattle PMID: 14594240
  37. association of four leptin gene polymorphisms in dairy cows (R4C, A59V, RFLP1, and BM1500) with circulating leptin concentrations during the periparturient period PMID: 14629116
  38. leptin is unable to accelerate the pulse generator of LH and GnRH in heifers at any developmental stage. PMID: 15128593
  39. Dysregulation of PL and leptin in nuclear transfer placentomes could affect cell migration and invasion and subsequently placental metabolism and transfer of nutrients to fetus, leading to increased placental and fetal macrosomia. PMID: 15306554
  40. leptin plays an important role in mammary gland lactogenesis and the expression of leptin requires the presence of prolactin PMID: 15375055
  41. study reports associations between SNP in the 5' untranslated promoter region of the leptin gene with serum leptin concentration, growth, BW, feed intake, feeding behavior, and carcass merit PMID: 15583038
  42. Important associations between SNP within the leptin gene with lean yield, fatness (fat yield and subcutaneous fat) and tenderness were detected. PMID: 16100055
  43. study shows that there is a relationship between the concentration of leptin in follicular fluid and atresia in small follicles PMID: 16363147
  44. analysis of CpG islands of bovine Leptin and POU5F1 genes in cloned bovine fetuses PMID: 16474211
  45. observations reveal an important linkage between pulsatile luteinizing hormone(LH) secretion and blood leptin concentrations during the early postpartum period in dairy cows PMID: 16840618
  46. Characterization of single nucleotide polymorphisms in LEP in relation to production traits. PMID: 16879354
  47. Sheep, goat and cattle R-banded chromosome preps, obtained from synchronized cell cultures, were used to FISH-map leptin and SLC26A2 genes on single chromosome bands. LEP maps on OAR4q32 and CHI4q32. Improved cytogenetic maps of BTA4/OAR4/CHI4. PMID: 16974077
  48. results suggest that plasma leptin levels in cows are inversely controlled at the transcription level by volatile fatty acids(VFA) and long-chain fatty acids (LCFA) PMID: 17011156
  49. Modulatory effect of leptin on luteal progesterone production in vitro. PMID: 17154301
  50. In conclusion, this study revealed significant differences in body weight and backfat growth parameters across leptin genotypes for a sample of commercially fed beef cattle. PMID: 17431048

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Subcellular Location Secreted
Protein Families Leptin family
Database Links

KEGG: bta:280836

STRING: 9913.ENSBTAP00000019853

UniGene: Bt.1939

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