Bovine Myoglobin(MB) ELISA kit

Code CSB-EL013529BO
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
myoglobin
Alternative Names
MB ELISA Kit; Myoglobin ELISA Kit
Abbreviation
MB
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue Homogenates
Detection Range
15.6 ng/mL-1000 ng/mL
Sensitivity
3.9 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 90
Range % 83-99
1:2 Average % 91
Range % 89-99
1:4 Average % 95
Range % 90-100
1:8 Average % 95
Range % 86-98
Recovery
The recovery of bovine MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 87-99
EDTA plasma (n=4) 95 90-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
1000 2.184 2.201 2.193 2.099
500 1.628 1.643 1.636 1.542
250 1.023 1.015 1.019 0.925
125 0.565 0.575 0.570 0.476
62.5 0.318 0.305 0.312 0.218
31.2 0.179 0.192 0.186 0.092
15.6 0.129 0.133 0.131 0.037
0 0.095 0.092 0.094  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Bovine Myoglobin(MB) ELISA Kit was designed for the quantitative measurement of Bovine Myoglobin(MB) protein in serum, plasma, tissue Homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.

Customer Reviews and Q&A

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 Q&A
Q:

Do you have both Beef and buffalo Myoglobin (MB) detection kit in ELISA format.Could you please discuss with your technical team for the following : 1. Cross-reactivity of Bovine MB kit for Buffalo MB. I am awaiting your response.

A:
Hi
Bovine Myoglobin(MB) ELISA kit,#CSB-EL013529BO This kit is specific to general bovine.We only test ordinary bovine.But there should be no big problems on Beef and buffalo.We suggest you do pre-testing first and see
Q:

Could you please revisit/discuss once more with your technical team for the following : 1. Coating Antibody is polyclonal - Yes or No. 2. Biotin Labeled antibody is polyclonal - Yes or No.
I am awaiting your response.

A:
Bovine Myoglobin(MB) ELISA kit,#CSB-EL013529BO This kit is specific to general bovine.We did not valiadate this kit on Buffalo.But there should be no big problems.We suggest you do pre-testing first and see

Target Background

Function
(From Uniprot)
Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
Gene References into Functions
  1. These results suggest an important role of genetics in meat color variation for cattle raised under the tropic conditions. PMID: 26946475
  2. Glycine at E14 in myoglobin enhances autoxidation and hemin loss rates. PMID: 22681513
Protein Families
Globin family
Database Links
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