MB

IHC image of CSB-RA283068A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA283068A0HU diluted at 1:100 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing PC-3 cells stained with CSB-RA283068A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Western Blot
All lanes: Mouse anti-Human Myoglobin monoclonal antibody at 0.3µg/ml
Lane 1: Mouse heart muscle lysate
Lane 2: Recombinant Myoglobin at 10µg
Secondary HRP labeled Goat polyclonal to Mouse IgG at 1/3000 dilution
Predicted band size: 18 kDa
Observed band size: 20 kDa
Additional bands at: 120 kDa

Western Blot
All lanes: Mouse anti-Human Myoglobin monoclonal antibody at 1µg/ml
Lane 1: Myoglobin transfected 293 cell lysate
Secondary HRP labeled Goat polyclonal to Mouse IgG at 1/3000 dilution
Predicted band size: 18 kDa
Observed band size: 20 kDa
Additional bands at: 23, 25, 40 kDa

Immunohistochemical of paraffin-embedded human heart tissue using CSB-MA079101A0m at dilution of 1:100

Immunofluorescent analysis of MCF-7 cells using CSB-MA079101A0m at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

Western Blot
Positive WB detected in: Mouse heart tissue, Mouse skeletal muscle tissue
All lanes: MB antibody at 1:2000
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa

Western blot
All lanes: MB antibody at 2µg/ml
Lane 1: Rat skeletal muscle tissue
Lane 2: Mouse heart tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa

Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA07917A0Rb at dilution of 1:100

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA07917A0Rb at dilution of 1:100