Bovine Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

Instructions
Code CSB-E14002B
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name prolactin
Alternative Names PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
Abbreviation PRL
Uniprot No. P01239
Species Bos taurus (Bovine)
Sample Types serum, plasma
Detection Range 20 μIU/mL-8000 μIU/mL
Sensitivity 20 μIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %91-100
1:2Average %95
Range %86-104
1:4Average %90
Range %85-95
1:8Average %96
Range %92-108
Recovery
The recovery of bovine PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9691-102
EDTA plasma (n=4)9589-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μIU/mlOD1OD2Average
80000.361 0.369 0.365
15000.695 0.704 0.700  
2501.205 1.199 1.202
701.524 1.520 1.522
201.703 1.709 1.706
02.245 2.243 2.244
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Prolactin acts primarily on the mammary gland by promoting lactation.
Gene References into Functions
  1. Taken together, these results support novel functions of prolactin as a modulator of the innate immune response that do not involve the classical prolactin pathway. PMID: 26341952
  2. The presence and localization of prolactin receptor are consistent with expression data reported for other species, and the presence of PIP and prolactin in seminal fluid is consistent with data generated in humans. PMID: 25533929
  3. Functionally reciprocal mutations of the prolactin signaling pathway define hairy and slick cattle. PMID: 25519203
  4. The single nucleotide polymorphism rs42646708 of cattle XKR4 was significantly associated with serum prolactin concentrations and explained 2.45% of the phenotypic variation. PMID: 24666329
  5. Five mutations were identified in exonic region and eleven in associated intronic regions in PRL gene in 100 cattle from four Pakistani cattle breeds. PMID: 24065524
  6. this study identifies a biochemical mechanism for the regulation of SCFAs on bovine GH and PRL gene transcription in dairy cow anterior pituitary cells PMID: 24177567
  7. These data suggest that PRL protects brain endothelial cells against methamphetamine-induced toxicity PMID: 23988027
  8. Bovine prolactin improved the expression of human transferrin through such a possible mechanism that bovine prolactin activated STAT5a transcription expression. PMID: 22829284
  9. There was no significant difference between 1134 locus and milk performance traits of 5'-UTR of PRL gene PMID: 22207382
  10. Staphylococcus aureus infection inhibits nuclear factor kappa B activation mediated by prolactin in bovine mammary epithelial cells. PMID: 21843629
  11. Study of genetic variation in Yakutian cattle (Bos taurus L.) using the prolactin bPRL, growth hormone bGH, and transcription factor bPit-1 genes PMID: 20391788
  12. This is the second study reporting single nucleotide polymorphisms in the 5'-regulatory region of PRL gene, which interfere with milk production traits. PMID: 19714484
  13. evidence for a functional coupling between the PRL salt bridge and phosphorylation site indicates that either in vivo phosphorylation or specific mutations that destabilize the salt bridge impair biological activity. PMID: 12850287
  14. determination of the effects of exposure to different lengths of daylight during the dry period on circulating PRL and PRL receptor mRNA expression in lymphocytes and mammary tissue during the transition to lactation PMID: 15591374
  15. Expression of PRL and prolactin receptor (PRLR) mRNA is demonstrated for the first time in bovine corpus luteum throughout the luteal phase. PMID: 16435374
  16. The A-->G transition at position -1043 abolishes the recognition site for Hsp92II restriction endonuclease. PMID: 17929163
  17. Prolactin in ovarian follicular fluid stimulates endothelial cell proliferation. PMID: 19672107

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Subcellular Location Secreted.
Protein Families Somatotropin/prolactin family
Database Links

KEGG: bta:280901

STRING: 9913.ENSBTAP00000020313

UniGene: Bt.7197

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