Bovine Serum amyloid A,SAA ELISA Kit

Code CSB-E08592b
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P35541
Lead Time 3-5 working days
Abbreviation SAA1
Protein Biological Process 1 Immunity
Target Name serum amyloid A1
Alias MGC111216, PIG4, SAA, TP53I4, tumor protein p53 inducible protein 4
Species Bos taurus (Bovine)
Protein Biological Process 3 Acute phase
Sample Types serum, plasma, tissue homogenates
Detection Range 0.1 μg/mL-20 μg/mL
Sensitivity 0.05 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
HGNC 10513
MGI 98221
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 88-96  
1:2 Average % 93  
Range % 89-98  
1:4 Average % 95  
Range % 91-98  
1:8 Average % 92  
Range % 90-95  
Recovery
The recovery of bovine SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-99  
EDTA plasma (n=4) 96 89-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
0 1.963 1.977 1.970    
0.1 1.644 1.654 1.649    
0.4 1.239 1.245 1.242    
1.6 0.817 0.798 0.808    
5 0.372 0.368 0.370    
20 0.121 0.123 0.122    
References
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Function Major acute phase reactant. Apolipoprotein of the HDL complex.
Involvement in disease Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function.
Subcellular Location Secreted
Protein Families SAA family
Tissue Specificity Expressed by the liver; secreted in plasma.
Database Links

KEGG: bta:506412

STRING: 9913.ENSBTAP00000030310

UniGene: Bt.88760

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