Bovine anti-Mullerian hormone (AMH) ELISA kit

Instructions
Code CSB-E13406B
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name anti-Mullerian hormone
Alternative Names AMH ELISA Kit; Muellerian-inhibiting factor ELISA Kit; Anti-Muellerian hormone ELISA Kit; AMH ELISA Kit; Muellerian-inhibiting substance ELISA Kit; MIS ELISA Kit
Abbreviation AMH
Uniprot No. P03972
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.5 ng/mL-200 ng/mL
Sensitivity 0.5 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine AMH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %90-101
1:2Average %98
Range %91-105
1:4Average %92
Range %88-97
1:8Average %97
Range %93-105
Recovery
The recovery of bovine AMH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-99
EDTA plasma (n=4)9790-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2Average
2000.134 0.139 0.137
37.50.211 0.203 0.207
6.250.495 0.485 0.490
1.750.968 0.987 0.978
0.51.146 1.154 1.150
02.226 2.250 2.238
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin.
Gene References into Functions
  1. Anti-Mullerian hormone inhibits activation and growth of bovine ovarian follicles in vitro and is localized to growing follicles. PMID: 28333275
  2. This study showed that antral follicle counts (AFC) and AMH levels were repeatable when determined at an unknown stage of follicular growth and expected day of follicular wave emergence, but repeatability was greater for AMH than AFC. PMID: 28237349
  3. The effects of castration and other surgical intervention on the blood levels of anti-Muellerian hormone, inhibin A, gonadotropins, and gonadotropin receptors in bull calves are reported. PMID: 27693012
  4. Results from these studies indicate that AMH signaling plays a role in both regulating granulosa cell proliferation and preventing granulosa cells from 5- to 8-mm follicles from undergoing premature differentiation before follicle selection. PMID: 27268296
  5. These findings indicate the followings: AMH mRNA levels decrease in both dominant and secondary follicles during follicular deviation; granulosa cells from heathy follicles express more AMH mRNA compared to subordinate follicles undergoing atresia and FSH stimulates AMH and AMHR2 mRNA expression in granulosa cells of co-dominant follicles. PMID: 26815645
  6. Measurement of AMH concentration in the plasma of cows can help to predict their capacity for embryo production in response to gonadotrophin treatment. PMID: 22935154
  7. In first study to investigate the blood profile and immunohuistochemistry of anti-Mullerian hormone in bovine granulosa-theca cell tumors, the findings indicated that anti-Mullerian hormone is a novel biomarker for granulosa-theca cell tumors in cattle. PMID: 22052009
  8. Regulation of anti-Mullerian hormone production in the cow. PMID: 21076084
  9. Intrafollicular AMH was not a marker of cystic development in the cow, but low AMH concentrations in cysts were associated with luteinization. PMID: 18448844
  10. AMH expression is modulated by androgens in bovine granulosa cells from small follicles. PMID: 19714502

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Subcellular Location Secreted
Protein Families TGF-beta family
Database Links

KEGG: bta:280718

STRING: 9913.ENSBTAP00000019912

UniGene: Bt.7050

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