Bovine follicle-stimulating hormone(FSH)ELISA Kit

Bovine follicle-stimulating hormone(FSH)ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Target Background

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

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Bovine follicle-stimulating hormone(FSH)ELISA Kit

Citations (5) Protocol

Code	CSB-E15856B
Size	96T,5×96T,10×96T
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Product Details
Citations (5)
Related Products
Customer Reviews and Q&A
Target Background

Target Name

follicle-stimulating hormone(FSH)

Alternative Names

FSHB ELISA Kit; Follitropin subunit beta ELISA Kit; Follicle-stimulating hormone beta subunit ELISA Kit; FSH-B ELISA Kit; FSH-beta ELISA Kit; Follitropin beta chain ELISA Kit

Abbreviation

FSH

Uniprot No.

P04837

Species

Bos taurus (Bovine)

Sample Types

serum, plasma, tissue homogenates

Detection Range

2 mIU/mL-800 mIU/mL

Sensitivity

2 mIU/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-105
1:2	Average %	93
	Range %	89-100
1:4	Average %	95
	Range %	89-100
1:8	Average %	97
	Range %	92-107

Recovery

The recovery of bovine FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-96
EDTA plasma (n=4)	94	90-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average
800	0.448	0.439	0.444
150	0.821	0.824	0.823
25	1.354	1.329	1.342
7	1.711	1.705	1.708
2	2.019	2.011	2.015
0	2.555	2.566	2.561

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

Researchers can use the Bovine follicle-stimulating hormone (FSH) ELISA Kit for quantitative detection of bovine FSH in serum, plasma, and tissue homogenates. The kit's detection range is 2 mIU/mL-800 mIU/mL, with a sensitivity of 2 mIU/mL. It employs a competitive detection mechanism, where the FSH in the sample competes with a labeled FSH for a limited number of antibody binding sites. This competition creates an inverse relationship between the concentration of FSH in the sample and the signal output, allowing for precise quantification. The assay requires 50-100 μL of sample and can be completed in 1-5 hours, with results measured at 450 nm wavelength. This bovine FSH ELISA kit has been used in the experimental setup of several papers.

Effect of a Single Administration of Fsh Delivered in Hyaluronic Acid on Oocyte Competence and Hormonal Concentrations in Italian Mediterranean Buffaloes Undergoing Ovarian Stimulation Prior to Ovum Pick-Up MA Kosior, V Longobardi, C Del Prete,SSRN,2025
Rumen-protected glucose stimulates the secretion of reproductive hormones and the mTOR/AKT signaling pathway in the ovaries of early postpartum Y Wang,researchsquare,2022
Bone Marrow-Derived Mesenchymal Stem Cells Reverse Radiotherapy-Induced Premature Ovarian Failure: Emphasis on Signal Integration of TGF-β, Wnt/β-Catenin and Hippo Pathways MO El-Derany,Stem Cell Reviews and Reports,2021
Regulation of AMH, AMHR-II, and BMPs (2,6) Genes of Bovine Granulosa Cells Treated with Exogenous FSH and Their Association with Protein Hormones Umer S, et al,Genes,2019
Fixed-time induction of ovulation in camels superovulated by different eCG modalities during the transition period in Egypt : Superovulation in camels during the transition period. Marwa Ahmed Khalifa.et al,Tropical Animal Health and Production,2016

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Function
(From Uniprot)

Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.

Gene References into Functions

Study describes generation and characterization of a new Fshb-iCre deleter strain using 4.7 kb of ovine Fshb promoter sequences driving iCre expression exclusively in the gonadotrope lineage with mice developing normally, and fertile. PMID: 26472536
the novel cis-regulatory element generated by the c.-1539_-1538delGGinsTTAACT mutations might play an important role in repressing FSHB transcription, possibly leading to reduced fertility PMID: 26478576
A 200-base region in the bovine FSHbeta 3' untranslated region downregulates reporter expression at the translational level via interactions between AREs in both the 3'UTR and transfactors. PMID: 15189830
The shift in FSH isoforms toward a greater abundance of the less acidic isoforms appears to be a key component in determining the capability for producing a preovulatory rise in estradiol. PMID: 18400447
The effect of a single nucleotide polymorphism of the follicle stimulating hormone beta chain on bull fertility and semen quality is reported. PMID: 18829190

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Subcellular Location

Secreted.

Protein Families

Glycoprotein hormones subunit beta family

Database Links

KEGG: bta:281171

STRING: 9913.ENSBTAP00000014462

UniGene: Bt.406

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Email: [email protected]
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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

TWIK2 channel mediates inflammation, study finds Effects of FSHR on Fertility and Tumors Tumor markers PI3K-Akt Signaling Pathway and Cancer Do You Really Know TGF-β Superfamily? ELISA Data Analysis How to Do The Pre-experiment before Your ELISA Experiment Four Types of ELISA 11 tips for choosing your right ELISA kit Do you Often Trouble in These Problems of ELISA? Problems and Solutions in ELISA Experiments

Related pathways

cAMP signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.