Bovine follicle-stimulating hormone(FSH)ELISA Kit

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Bovine follicle-stimulating hormone(FSH)ELISA Kit

Citations (4) Protocol

Code	CSB-E15856B
Size	96T,5×96T,10×96T
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Product Details
Citations (4)
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Description

This Bovine FSH ELISA Kit was designed for the quantitative measurement of Bovine FSH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 2 mIU/mL-800 mIU/mL and the sensitivity is 2 mIU/mL.

Target Name

follicle-stimulating hormone(FSH)

Alternative Names

FSHB ELISA Kit; Follitropin subunit beta ELISA Kit; Follicle-stimulating hormone beta subunit ELISA Kit; FSH-B ELISA Kit; FSH-beta ELISA Kit; Follitropin beta chain ELISA Kit

Abbreviation

FSH

Uniprot No.

P04837

Species

Bos taurus (Bovine)

Sample Types

serum, plasma, tissue homogenates

Detection Range

2 mIU/mL-800 mIU/mL

Sensitivity

2 mIU/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-105
1:2	Average %	93
	Range %	89-100
1:4	Average %	95
	Range %	89-100
1:8	Average %	97
	Range %	92-107

Recovery

The recovery of bovine FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-96
EDTA plasma (n=4)	94	90-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average
800	0.448	0.439	0.444
150	0.821	0.824	0.823
25	1.354	1.329	1.342
7	1.711	1.705	1.708
2	2.019	2.011	2.015
0	2.555	2.566	2.561

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Rumen-protected glucose stimulates the secretion of reproductive hormones and the mTOR/AKT signaling pathway in the ovaries of early postpartum Y Wang,researchsquare,2022
Bone Marrow-Derived Mesenchymal Stem Cells Reverse Radiotherapy-Induced Premature Ovarian Failure: Emphasis on Signal Integration of TGF-β, Wnt/β-Catenin and Hippo Pathways MO El-Derany,Stem Cell Reviews and Reports,2021
Regulation of AMH, AMHR-II, and BMPs (2,6) Genes of Bovine Granulosa Cells Treated with Exogenous FSH and Their Association with Protein Hormones Umer S, et al,Genes,2019
Fixed-time induction of ovulation in camels superovulated by different eCG modalities during the transition period in Egypt : Superovulation in camels during the transition period. Marwa Ahmed Khalifa.et al,Tropical Animal Health and Production,2016

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Function (From Uniprot)	Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.
Gene References into Functions	Study describes generation and characterization of a new Fshb-iCre deleter strain using 4.7 kb of ovine Fshb promoter sequences driving iCre expression exclusively in the gonadotrope lineage with mice developing normally, and fertile. PMID: 26472536 the novel cis-regulatory element generated by the c.-1539_-1538delGGinsTTAACT mutations might play an important role in repressing FSHB transcription, possibly leading to reduced fertility PMID: 26478576 A 200-base region in the bovine FSHbeta 3' untranslated region downregulates reporter expression at the translational level via interactions between AREs in both the 3'UTR and transfactors. PMID: 15189830 The shift in FSH isoforms toward a greater abundance of the less acidic isoforms appears to be a key component in determining the capability for producing a preovulatory rise in estradiol. PMID: 18400447 The effect of a single nucleotide polymorphism of the follicle stimulating hormone beta chain on bull fertility and semen quality is reported. PMID: 18829190 Show More Hide All
Subcellular Location	Secreted.
Protein Families	Glycoprotein hormones subunit beta family
Database Links	KEGG: bta:281171 STRING: 9913.ENSBTAP00000014462 UniGene: Bt.406

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Related pathways

cAMP signaling pathway

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

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