Bovine follicle-stimulating hormone(FSH)ELISA Kit

Instructions
Code CSB-E15856B
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name follicle-stimulating hormone(FSH)
Alternative Names FSHB ELISA Kit; Follitropin subunit beta ELISA Kit; Follicle-stimulating hormone beta subunit ELISA Kit; FSH-B ELISA Kit; FSH-beta ELISA Kit; Follitropin beta chain ELISA Kit
Abbreviation FSH
Uniprot No. P04837
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 2 mIU/mL-800 mIU/mL
Sensitivity 2 mIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 91-105  
1:2 Average % 93  
Range % 89-100  
1:4 Average % 95  
Range % 89-100  
1:8 Average % 97  
Range % 92-107  
Recovery
The recovery of bovine FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 89-96  
EDTA plasma (n=4) 94 90-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/ml OD1 OD2 Average    
800 0.448 0.439 0.444    
150 0.821 0.824 0.823    
25 1.354 1.329 1.342    
7 1.711 1.705 1.708    
2 2.019 2.011 2.015    
0 2.555 2.566 2.561    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Stimulates development of follicle and spermatogenesis in the reproductive organs.
Gene References into Functions
  1. Study describes generation and characterization of a new Fshb-iCre deleter strain using 4.7 kb of ovine Fshb promoter sequences driving iCre expression exclusively in the gonadotrope lineage with mice developing normally, and fertile. PMID: 26472536
  2. the novel cis-regulatory element generated by the c.-1539_-1538delGGinsTTAACT mutations might play an important role in repressing FSHB transcription, possibly leading to reduced fertility PMID: 26478576
  3. A 200-base region in the bovine FSHbeta 3' untranslated region downregulates reporter expression at the translational level via interactions between AREs in both the 3'UTR and transfactors. PMID: 15189830
  4. The shift in FSH isoforms toward a greater abundance of the less acidic isoforms appears to be a key component in determining the capability for producing a preovulatory rise in estradiol. PMID: 18400447
  5. The effect of a single nucleotide polymorphism of the follicle stimulating hormone beta chain on bull fertility and semen quality is reported. PMID: 18829190

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Subcellular Location Secreted
Protein Families Glycoprotein hormones subunit beta family
Database Links

KEGG: bta:281171

STRING: 9913.ENSBTAP00000014462

UniGene: Bt.406

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