Bovine follicle-stimulating hormone(FSH)ELISA Kit

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Target Data

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Bovine follicle-stimulating hormone(FSH)ELISA Kit

Name: Bovine follicle-stimulating hormone(FSH)ELISA Kit
Brand: CUSABIO
SKU: CSB-E15856B

^Instructions

Code	CSB-E15856B
Size	96T,5×96T,10×96T

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Target Data

Target Name

follicle-stimulating hormone(FSH)

Alternative Names

N/A

Abbreviation

FSH

Uniprot No.

P04837

Species

Bos taurus (Bovine)

Sample Types

serum, plasma, tissue homogenates

Detection Range

2 mIU/mL-800 mIU/mL

Sensitivity

2 mIU/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-105
1:2	Average %	93
	Range %	89-100
1:4	Average %	95
	Range %	89-100
1:8	Average %	97
	Range %	92-107

Recovery

The recovery of bovine FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-96
EDTA plasma (n=4)	94	90-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average
800	0.448	0.439	0.444
150	0.821	0.824	0.823
25	1.354	1.329	1.342
7	1.711	1.705	1.708
2	2.019	2.011	2.015
0	2.555	2.566	2.561

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Regulation of AMH, AMHR-II, and BMPs (2,6) Genes of Bovine Granulosa Cells Treated with Exogenous FSH and Their Association with Protein Hormones Umer S, et al,Genes,2019
Fixed-time induction of ovulation in camels superovulated by different eCG modalities during the transition period in Egypt : Superovulation in camels during the transition period. Marwa Ahmed Khalifa.et al,Tropical Animal Health and Production,2016

Function	Stimulates development of follicle and spermatogenesis in the reproductive organs.
Subcellular Location	Secreted
Protein Families	Glycoprotein hormones subunit beta family
Database Links	KEGG: bta:281171 STRING: 9913.ENSBTAP00000014462 UniGene: Bt.406

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

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