Bovine neuropeptide Y (NPY) ELISA kit

Code CSB-E08171b
Size 96T,5×96T,10×96T
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Product Details

Target Name
neuropeptide Y
Alternative Names
NPY ELISA Kit; Pro-neuropeptide Y [Cleaved into: Neuropeptide Y ELISA Kit; Neuropeptide tyrosine ELISA Kit; NPY); C-flanking peptide of NPY ELISA Kit; CPON)] ELISA Kit
Abbreviation
NPY
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
serum, plasma
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine NPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %87-93
1:2Average %102
Range %98-105
1:4Average %90
Range %86-96
1:8Average %91
Range %88-97
Recovery
The recovery of bovine NPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9086-94
EDTA plasma (n=4)9187-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.604 2.490 2.547 2.444
102.113 2.221 2.167 2.064
51.657 1.711 1.684 1.581
2.51.114 1.056 1.085 0.982
1.250.712 0.687 0.700 0.597
0.6250.413 0.428 0.421 0.318
0.3120.278 0.260 0.269 0.166
00.105 0.101 0.103
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Bovine NPY ELISA Kit was designed for the quantitative measurement of Bovine NPY protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

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Target Background

Function
(From Uniprot)
NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
Gene References into Functions
  1. Hormonal gene expression involved in residual feed intake in dairy cows may be related to the molecular regulation of the leptin-NPY and insulin signaling pathways. PMID: 26231801
  2. Data indicate that increased body weight gain during juvenile development accelerates sexual maturation in heifers, coincident with reciprocal changes in circulating concentrations of leptin and hypothalamic neuropeptide Y (NPY) release. PMID: 25326602
  3. There is a strong association between putative favorable allelic variants (SNP) of neuropeptide Y, leptin, and IGF-1 genes, and residual feed intake, when animals were grazing on a high-quality, high-availability pasture. PMID: 23881687
  4. The NPY SNP (NPY1) was associated with the prevalence of the animal being in calf 100 d after calving and 305-d milk yield in the first lactation. PMID: 21700051
  5. NPY tended to increase serum growth hormone, which appeared to be a consequence of increased pulse amplitude. Infusion of NPY also increased CSF growth hormone-releasing hormone. PMID: 19632078
  6. The effect of single nucleotide polymorphisms in 6 genes and their associations with production factors in beef cattle are reported. PMID: 17785604
  7. Data show that PKA and PKC pathways are involved in the differential regulation of production of the neuropeptides (Met)enkephalin, galanin, somatostatin, NPY, and VIP. PMID: 18619673

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Subcellular Location
Secreted. Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle.
Protein Families
NPY family
Database Links
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