Canine Dihydrotestosterone,DHT ELISA Kit

Code CSB-E07881c
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Dihydrotestosterone,DHT
Abbreviation
DHT
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
40 pg/mL-8000 pg/mL
Sensitivity
20 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine DHT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %97
Range %93-103
1:2Average %95
Range %91-99
1:4Average %102
Range %98-104
1:8Average %89
Range %82-95
Recovery
The recovery of canine DHT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-99
EDTA plasma (n=4)9998-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
80000.224 0.223 0.224
20000.362 0.356 0.359
6400.564 0.557 0.561
1600.819 0.804 0.812
401.174 1.275 1.225
02.305 2.404 2.355
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Dog DHT ELISA Kit was designed for the quantitative measurement of Dog DHT protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 40 pg/mL-8000 pg/mL and the sensitivity is 20 pg/mL .

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