Canine Dihydrotestosterone,DHT ELISA Kit

Code CSB-E07881c
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation DHT
Target Name Dihydrotestosterone,DHT
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, tissue homogenates
Detection Range 40 pg/mL-8000 pg/mL
Sensitivity 20 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of canine DHT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %97
Range %93-103
1:2Average %95
Range %91-99
1:4Average %102
Range %98-104
1:8Average %89
Range %82-95
The recovery of canine DHT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-99
EDTA plasma (n=4)9998-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
80000.224 0.223 0.224
20000.362 0.356 0.359
6400.564 0.557 0.561
1600.819 0.804 0.812
401.174 1.275 1.225
02.305 2.404 2.355
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