Canine Dihydrotestosterone,DHT ELISA Kit

Canine Dihydrotestosterone,DHT ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

Related Products

Customer Reviews and Q&A

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Canine Dihydrotestosterone,DHT ELISA Kit

Citations (2) Protocol

Code	CSB-E07881c
Size	96T,5×96T,10×96T
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Product Details
Citations (2)
Related Products
Customer Reviews and Q&A

Target Name

Dihydrotestosterone,DHT

Abbreviation

DHT

Species

Canis lupus familiaris (Dog) (Canis familiaris)

Sample Types

serum, plasma, tissue homogenates

Detection Range

40 pg/mL-8000 pg/mL

Sensitivity

20 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of canine DHT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	93-103
1:2	Average %	95
	Range %	91-99
1:4	Average %	102
	Range %	98-104
1:8	Average %	89
	Range %	82-95

Recovery

The recovery of canine DHT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	99	98-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
8000	0.224	0.223	0.224
2000	0.362	0.356	0.359
640	0.564	0.557	0.561
160	0.819	0.804	0.812
40	1.174	1.275	1.225
0	2.305	2.404	2.355

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Dog DHT ELISA Kit was designed for the quantitative measurement of Dog DHT protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 40 pg/mL-8000 pg/mL and the sensitivity is 20 pg/mL .

Finasteride accelerates prostate wound healing after thulium laser resection through DHT and AR signalling Zhao R.et al,Cell Prolif,2017
Androgen Deprivation Accelerates the Prostatic Urethra Wound Healing After Thulium Laser Resection of the Prostate by Promoting Re-Epithelialization and Regulating the Macrophage Polarization Xing-Jie Wang.et al,THE PROSTATE,2017

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.