Rat Dihydrotestosterone,DHT ELISA Kit

Code CSB-E07879r
Size 96T,5×96T,10×96T
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Product Details

Target Name
Dihydrotestosterone,DHT
Alternative Names
N/A
Abbreviation
DHT
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
10 pg/mL-2000 pg/mL
Sensitivity
5 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat DHT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 102  
Range % 98-106  
1:2 Average % 93  
Range % 89-97  
1:4 Average % 95  
Range % 89-99  
1:8 Average % 102  
Range % 98-106  
Recovery
The recovery of rat DHT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-95  
EDTA plasma (n=4) 102 97-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
2000 0.156 0.160 0.158    
500 0.312 0.329 0.321    
160 0.498 0.528 0.513    
40 0.818 0.787 0.803    
10 1.316 1.278 1.297    
0 1.960 1.954 1.957    
Materials provided
  • A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit DHT antibody.
  • Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
  • Anti-mouse DHT Antibody 1 x 6 ml -- Act as the detection antibody.
  • HRP-conjugate 1 x 6 ml -- Bind to the DHT antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
  • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
  • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
  • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
  • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells)
  • An Instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat DHT ELISA Kit was designed for the quantitative measurement of Rat DHT protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 10 pg/mL-2000 pg/mL and the sensitivity is 5 pg/mL.

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