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Canine Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

Protocol
Code CSB-E14930c
Size 96T,5×96T,10×96T
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Product Details

Target Name
Prolactin/Luteotropic Hormone(PRL/LTH)
Abbreviation
PRL/LTH
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
7.5 μIU/mL-3000 μIU/mL
Sensitivity
7.5 μIU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %94
Range %91-100
1:2Average %90
Range %83-104
1:4Average %92
Range %89-100
1:8Average %96
Range %91-105
Recovery
The recovery of canine PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9592-98
EDTA plasma (n=4)9489-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μIU/mlOD1OD2Average
30000.405 0.408 0.407
562.50.733 0.726 0.730
93.751.198 1.195 1.197
26.251.479 1.484 1.482
7.51.621 1.626 1.624
02.445 2.446 2.446
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Dog PRL/LTH ELISA Kit was designed for the quantitative measurement of Dog PRL/LTH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 7.5 μIU/mL-3000 μIU/mL and the sensitivity is 7.5 μIU/mL .

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