Chicken Corticosterone, CORT ELISA kit

Chicken Corticosterone, CORT ELISA kit

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Chicken Corticosterone, CORT ELISA kit

Name: Chicken Corticosterone, CORT ELISA kit
Brand: CUSABIO
SKU: CSB-E11991C

^Instructions

Code	CSB-E11991C
Size	96T,5×96T,10×96T
Trial Size
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Target Name

Corticosterone, CORT

Abbreviation

CORT

Species

Gallus gallus (Chicken)

Sample Types

serum, plasma

Detection Range

0.5 ng/mL-20 ng/mL

Sensitivity

0.5 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-103
1:2	Average %	97
	Range %	91-101
1:4	Average %	90
	Range %	85-94
1:8	Average %	93
	Range %	87-98

Recovery

The recovery of chicken CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	95	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
20	0.161	0.149	0.155
10	0.245	0.259	0.252
5	0.408	0.394	0.401
2	0.658	0.609	0.634
1	0.959	0.904	0.932
0.5	1.341	1.276	1.309
0	1.842	1.772	1.807

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Dietary supplementation of Solanum aethiopicum and Solanecio biafrae leaves alters stress and immune responses, antioxidant status, and meat quality in broilers raised in a hot-dry environment K Adeyemi,British poultry science,2021
Research Note: A sip of stress. Effects of corticosterone supplementation in drinking water on feather corticosterone concentrations in layer pullets T Bartels,Poultry Science,2021
Effects of Deoxynivalenol-Contaminated Diets on Metabolic and Immunological Parameters in Broiler Chickens. Animals 2021, 11, 147 I Riahi,Animals,2021
Effects of flaxseed supplementation on omega-6 to omega-3 fatty acid balance, lipid mediator profile, proinflammatory cytokines and stress indices in laying hens SM Le,researchsquare,2020
Newcastle Disease Virus Induced Pathologies Severely Affect the Exocrine and Endocrine Functions of Pancreas in Chickens ZU Rehman,Research Square,2020
Chitosan oligosaccharide as an effective feed additive to maintain growth performance, meat quality, muscle glycolytic metabolism, and oxidative status in yellow-feather broilers under heat stress Q Chang,Poultry Science,2020
Orthosiphon stamineus and rosmarinic acid reduce heat stress in laying hens X Cai,Livestock Science,2020
Weeping forsythia extract alleviates dexamethasone-induced oxidative injury of breast muscles in broilers Pan L,Animal,2019
Attenuating effects of guanidinoacetic acid on preslaughter transport-induced muscle energy expenditure and rapid glycolysis of broilers Zhang L, et al,Poultry Science,2019
Dietary L-theanine alleviated lipopolysaccharide-induced immunological stress in yellow-feathered broilers RuiLi.et al,Animal Nutrition,2018
Forsythia suspensa extract protects broilers against breast muscle oxidative injury induced by corticosterone mimicked pre-slaughter acute stress Pan L.et al,Poult Sci,2018
Forsythia suspensa extract attenuates breast muscle oxidative injury induced by transport stress in broilers Pan L.et al,Poult Sci,2018
Creatine Monohydrate Enhances Energy Status and Reduces Glycolysis via Inhibition of AMPK Pathway in Pectoralis Major Muscle of Transport-Stressed Broilers Zhang L.et al,J Agric Food Chem.,2017

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.