Rabbit Corticosterone,CORT ELISA Kit

Code CSB-E06957Rb
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Alternative Names N/A
Abbreviation CORT
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma
Detection Range 0.5 ng/mL-20 ng/mL
Sensitivity 0.5 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 93    
Range % 85-105    
1:2 Average % 95    
Range % 85-105    
1:4 Average % 89    
Range % 83-98    
1:8 Average % 90    
Range % 83-99    
Recovery
The recovery of rabbit CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 97 90-110    
EDTA plasma (n=4) 96 91-100    
               
               
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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