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Rabbit Corticosterone,CORT ELISA Kit

Rabbit Corticosterone,CORT ELISA Kit

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Code	CSB-E06957Rb
Size	96T,5×96T,10×96T
Trial Size
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Alternative Names

N/A

Abbreviation

CORT

Species

Oryctolagus cuniculus (Rabbit)

Sample Types

serum, plasma

Detection Range

0.5 ng/mL-20 ng/mL

Sensitivity

0.5 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	85-105
1:2	Average %	95
	Range %	85-105
1:4	Average %	89
	Range %	83-98
1:8	Average %	90
	Range %	83-99

Recovery

The recovery of rabbit CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-110
EDTA plasma (n=4)	96	91-100

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Citations

Effect of High Levels of Ammonia in Air on Adrenal Response to Adrenocorticotropin and Forced Running in Rabbits Yanchev I.et al,/,2015

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Rabbit Corticosterone,CORT ELISA Kit

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