- A micro ELISA plate --- The 96-well plate has been pre-coated with goat anti-rabbit CORT antibody.
- Six vials lyophilized standard (0.5 ml/bottle) --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- Anti-mouse CORT Antibody (1 x 6 ml) --- Act as the detection antibody.
- HRP-conjugate (1 x 6 ml) --- HRP catalyzes TMB substrate to elicit a chromogenic reaction.
- Wash Buffer (20 x concentrate) (1 x 15 ml) --- Wash away unbound or free substances.
- Substrate A (1 x 7 ml) --- Mix with substrate B, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
- Substrate B (1 x 7 ml) --- Mix with substrate A, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
- Stop Solution 1 x 7 ml --- Stop the color development, and the eventual solution color turns yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microwell plate when incubation.
- An Instruction manual