Mouse Corticosterone,CORT ELISA Kit

Code CSB-E07969m
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse CORT ELISA Kit was designed for the quantitative measurement of Mouse CORT protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.1 ng/mL-20 ng/mL and the sensitivity is 0.05 ng/mL.

Alternative Names N/A
Abbreviation CORT
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 5 ng/ml-1000 ng/ml.
Sensitivity 2.5 ng/ml
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Materials provided
    • A micro ELISA plate --- The 96-well plate has been pre-coated with goat anti-rabbit CORT antibody.
    • Six vials lyophilized standard (0.5 ml/bottle) --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • Anti-mouse CORT Antibody (1 x 6 ml) --- Act as the detection antibody.
    • HRP-conjugate (1 x 6 ml) --- HRP catalyzes TMB substrate to elicit a chromogenic reaction.
    • Wash Buffer (20 x concentrate) (1 x 15 ml) --- Wash away unbound or free substances.
    • Substrate A (1 x 7 ml) --- Mix with substrate B, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
    • Substrate B (1 x 7 ml) --- Mix with substrate A, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
    • Stop Solution 1 x 7 ml --- Stop the color development, and the eventual solution color turns yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microwell plate when incubation.
    • An Instruction manual
Materials not provided
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm to 630 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 1 reviews

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Sample type: Tissue homogenate(brain)

Sample species: Mouse

Sample dilution: No dilution

Review: We used the Mouse Corticosterone ELISA kit for serum and brain homogenates of stressed and control mice and got pretty nice results with expected group differences. The calibration curve looked very good, and we did not have problems to reproduce the results. Differences in the absorbance in duplicates were very moderate.

By Anonymous

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