Mouse Corticosterone,CORT ELISA Kit

Code CSB-E07969m
Size 96T,5×96T,10×96T
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Product Details

Alternative Names
N/A
Abbreviation
CORT
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 ng/ml-1000 ng/ml.
Sensitivity
3.9 ng/ml.
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Sample

Serum(n=4)

1:1

Average %

84

Range %

80-92

1:2

Average %

87

Range %

81-95

1:4

Average %

85

Range %

82-93

1:8

Average %

88

Range %

82-98

Recovery

The recovery of mouse CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

90

86-98

EDTA plasma (n=4)

88

83-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

1000

0.150

0.144

0.147

500

0.185

0.182

0.183

250

0.261

0.248

0.254

125

0.389

0.400

0.395

62.5

0.642

0.621

0.632

31.25

1.004

1.053

1.028

15.6

1.410

1.384

1.397

0

2.166

2.125

2.145

Materials provided
    • A micro ELISA plate --- The 96-well plate has been pre-coated with goat anti-rabbit CORT antibody.
    • Six vials lyophilized standard (0.5 ml/bottle) --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • Anti-mouse CORT Antibody (1 x 6 ml) --- Act as the detection antibody.
    • HRP-conjugate (1 x 6 ml) --- HRP catalyzes TMB substrate to elicit a chromogenic reaction.
    • Wash Buffer (20 x concentrate) (1 x 15 ml) --- Wash away unbound or free substances.
    • Substrate A (1 x 7 ml) --- Mix with substrate B, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
    • Substrate B (1 x 7 ml) --- Mix with substrate A, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
    • Stop Solution 1 x 7 ml --- Stop the color development, and the eventual solution color turns yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microwell plate when incubation.
    • An Instruction manual
Materials not provided
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm to 630 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description
This Mouse CORT ELISA Kit was designed for the quantitative measurement of Mouse CORT protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 15.6 ng/ml-1000 ng/ml and the sensitivity is 3.9 ng/mL.

Citations

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 3 reviews

Submit a Review here

Sample type: Tissue homogenate(brain)

Sample species: Mouse

Sample dilution: No dilution

Review: We used the Mouse Corticosterone ELISA kit for serum and brain homogenates of stressed and control mice and got pretty nice results with expected group differences. The calibration curve looked very good, and we did not have problems to reproduce the results. Differences in the absorbance in duplicates were very moderate.

By Anonymous

Sample type: Serum

Sample species: Mouse

Sample dilution: 1:25

Review: The serum CORT levels of mice were detected by CSB-E07969m kit, and most of them were within the standard curve range after 25 times dilution, but 1:10 dilution can be considered if the sample is sufficient. All experiments were completed within 3.5 hours. The results were stable and the standard curves were good.

By Anonymous

Sample type: Serum

Sample species: Mouse

Sample dilution: 1:5

Review: It is as easy to use as the ACTH box, and the mouse serum is still within the detection range of standard melody after 5 times dilution, which saves samples and is extremely friendly to precious samples. The test time is very short, less than 3 hours to complete, it is recommended to do a pre-experiment to test the dilution ratio. The OD values of samples after 5-fold dilution of CORT mouse serum ranged from 0.627 to 1.581.

By Anonymous

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