Mouse Corticosterone,CORT ELISA Kit

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Name: Mouse Corticosterone,CORT ELISA Kit
Brand: CUSABIO
SKU: CSB-E07969m

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Code	CSB-E07969m
Size	96T,5×96T,10×96T
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Target Name

Corticosterone,CORT

Alternative Names

N/A

Abbreviation

CORT

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.1 ng/mL-20 ng/mL

Sensitivity

0.05 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	90
	Range %	85-95
1:4	Average %	87
	Range %	82-93
1:8	Average %	95
	Range %	90-101

Recovery

The recovery of mouse CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-104
EDTA plasma (n=4)	92	88-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
20	0.128	0.122	0.125
5	0.238	0.229	0.234
1.6	0.424	0.459	0.442
0.4	0.921	0.959	0.940
0.1	1.524	1.619	1.572
0	2.354	2.219	2.287

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

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