Mouse Corticosterone,CORT ELISA Kit

The Mouse Corticosterone (CORT) ELISA Kit is specifically designed to standardize the detection of mouse CORT in multiple biological solutions, including serum, plasma, and tissue homogenates. This kit employs the Competitive-ELISA mechanism to quantitatively measure the concentration of CORT in the sample. The microtiter plate has been pre-coated with the goat anti-rabbit CORT antibody. Standards or samples are pipetted into the microtiter plate with the anti-mouse CORT antibody and HRP-conjugate. A competitive inhibition reaction is launched between CORT (standards or samples) and HRP-conjugated CORT with the anti-mouse CORT antibody. And these Ag-Ab complexes are captured by the antibody immobilized on the plate. Following a wash to remove any unbound reagent, substrate A and B solutions are added to the wells to elicit a chromogenic reaction. The color development is terminated after adding the stop solution, and the solution color changes from blue to yellow. The color intensity is inversely proportional to the amount of CORT captured in the initial step and is measured by a microtiter plate reader at 450 nm.

This kit has been professionally verified with reliable quality, high sensitivity, strong specificity, good linearity, precision less than 10%, recovery of 88%-104%, and consistency between batches. See more details on the product instructions.

CORT is the primary avian glucocorticoid hormone. It positively signals the brain for many functions, but excess CORT may interfere with natural neuroplasticity. CORT plays a regulatory role in the intensity of licking behavior, which differs as a function of the endocrine background of the animal.