Mouse Corticosterone,CORT ELISA Kit

Code CSB-E07969m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

The Mouse Corticosterone (CORT) ELISA Kit is specifically designed to standardize the detection of mouse CORT in multiple biological solutions, including serum, plasma, and tissue homogenates. This kit employs the Competitive-ELISA mechanism to quantitatively measure the concentration of CORT in the sample. The microtiter plate has been pre-coated with the goat anti-rabbit CORT antibody. Standards or samples are pipetted into the microtiter plate with the anti-mouse CORT antibody and HRP-conjugate. A competitive inhibition reaction is launched between CORT (standards or samples) and HRP-conjugated CORT with the anti-mouse CORT antibody. And these Ag-Ab complexes are captured by the antibody immobilized on the plate. Following a wash to remove any unbound reagent, substrate A and B solutions are added to the wells to elicit a chromogenic reaction. The color development is terminated after adding the stop solution, and the solution color changes from blue to yellow. The color intensity is inversely proportional to the amount of CORT captured in the initial step and is measured by a microtiter plate reader at 450 nm.

This kit has been professionally verified with reliable quality, high sensitivity, strong specificity, good linearity, precision less than 10%, recovery of 88%-104%, and consistency between batches. See more details on the product instructions.

CORT is the primary avian glucocorticoid hormone. It positively signals the brain for many functions, but excess CORT may interfere with natural neuroplasticity. CORT plays a regulatory role in the intensity of licking behavior, which differs as a function of the endocrine background of the animal.

Alternative Names N/A
Abbreviation CORT
Species Mus musculus (Mouse)
Sample Types
Detection Range
Sensitivity
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Materials provided
    • A micro ELISA plate --- The 96-well plate has been pre-coated with goat anti-rabbit CORT antibody.
    • Six vials lyophilized standard (0.5 ml/bottle) --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • Anti-mouse CORT Antibody (1 x 6 ml) --- Act as the detection antibody.
    • HRP-conjugate (1 x 6 ml) --- HRP catalyzes TMB substrate to elicit a chromogenic reaction.
    • Wash Buffer (20 x concentrate) (1 x 15 ml) --- Wash away unbound or free substances.
    • Substrate A (1 x 7 ml) --- Mix with substrate B, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
    • Substrate B (1 x 7 ml) --- Mix with substrate A, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
    • Stop Solution 1 x 7 ml --- Stop the color development, and the eventual solution color turns yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microwell plate when incubation.
    • An Instruction manual
Materials not provided
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm to 630 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

 Customer Reviews
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Sample type: Tissue homogenate(brain)

Sample species: Mouse

Sample dilution: No dilution

Review: We used the Mouse Corticosterone ELISA kit for serum and brain homogenates of stressed and control mice and got pretty nice results with expected group differences. The calibration curve looked very good, and we did not have problems to reproduce the results. Differences in the absorbance in duplicates were very moderate.

By Anonymous

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  Email: [email protected]
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