Chicken Immunoglobulin A,IgA ELISA Kit

Code CSB-E11232Ch
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation IgA
Target Name Immunoglobulin A,IgA
Species Gallus gallus (Chicken)
Sample Types serum, plasma
Detection Range 781.25 ng/mL-50000 ng/mL
Sensitivity 390 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 97  
Range % 93-105  
1:200 Average % 94  
Range % 91-98  
1:400 Average % 102  
Range % 98-106  
1:800 Average % 88  
Range % 82-96  
Recovery
The recovery of chicken IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 92-102  
EDTA plasma (n=4) 102 98-108  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
50000 0.072 0.071 0.072    
25000 0.134 0.136 0.135    
12500 0.231 0.242 0.237    
6250 0.426 0.435 0.431    
3125 0.666 0.675 0.671    
1562.5 0.938 0.927 0.933    
781.25 1.248 1.147 1.198    
0 1.695 1.594 1.645    
References
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Q&A and Customer Reviews

Q&A
Q:

For the kit CSB-E11232Ch, a customer asks if they have been tested with tissue samples, specifically small intestine.
Would you have this information available to share?

A:
Thanks for your inquiry.
We suggest you do a pretest if you want to test small intestine.Pls let me know if you have any further questions. Thank you.
Customer Reviews

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