Pig Immunoglobulin A,IgA ELISA Kit

Code CSB-E13234p
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation IgA
Target Name Immunoglobulin A,IgA
Species Sus scrofa (Pig)
Sample Types serum, plasma
Detection Range 0.146 μg/mL-37.5 μg/mL
Sensitivity 0.146 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1000 Average % 92  
Range % 88-94  
1:2000 Average % 93  
Range % 90-98  
1:4000 Average % 88  
Range % 85-94  
1:8000 Average % 95  
Range % 91-100  
Recovery
The recovery of pig IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-97  
EDTA plasma (n=4) 103 98-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
37.5 0.199 0.209 0.204    
9.375 0.445 0.420 0.433    
2.345 0.924 0.955 0.940    
0.586 1.413 1.447 1.430    
0.146 2.101 2.114 2.108    
0 2.547 2.503 2.525    
References
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Q&A and Customer Reviews

Q&A
Q:

Hi. I would like to know if this kit is used feces samples from pig. If so, I would like knowing the cost. Also I would like to know if you have avilable the kit with reagents for plate preparation and its respective cost, as well. Thanks much.

A:
Thanks for your inquiry.
For IgA, there are serotype and secretory type. If you want to test feces samples, we recommend you choose Pig secretory immunoglobulin A,SIgA ELISA KitCSB-E12063p. No matter you use CSB-E12063p or CSB-E13234p, we suggest you do a pretest first.
All the components in the kit are well prepared, so you don't need to prepare the reagents by yourself. Sorry we can not provide reagents for plate preparation.Pls let me know if you have any further questions. Thank you.
Customer Reviews

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