Human Immunoglobulin A,IgA ELISA Kit

Code CSB-E07985h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details

Target Name
Immunoglobulin A,IgA
Alternative Names
Homo sapiens (Human)
Sample Types
serum, plasma
Detection Range
0.24 ng/mL-1000 ng/mL
0.24 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:10000 Average % 89  
Range % 85-94  
1:20000 Average % 90  
Range % 86-97  
1:40000 Average % 96  
Range % 90-103  
1:80000 Average % 89  
Range % 86-93  
The recovery of human IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 89-94  
EDTA plasma (n=4) 92 88-97  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
1000 2.180 2.205 2.193 2.124  
250 2.093 2.002 2.048 1.979  
62.5 1.841 1.868 1.855 1.786  
15.6 1.528 1.501 1.515 1.446  
3.9 0.772 0.753 0.763 0.694  
0.98 0.396 0.397 0.397 0.328  
0.24 0.189 0.180 0.185 0.116  
0 0.069 0.068 0.069    
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This human IgA ELISA Kit is suitable for qualitatively determining human IgA concentrations in the serum and plasma in vitro. IgA makes up more than two-thirds of the total immunoglobulin produced by the body. IgA acts as a first-line barrier, preventing intestinal antigens from reaching the gut mucosa, regulating the intestinal microbiota, and dampening pro-inflammatory immune responses. IgA also removes pathogens or antigens by an IgA-mediated excretory pathway, in which immune complexes are bound to IgA and then transported by polyimmunoglobulin receptors. At mucosal surfaces, secretory IgA plays a key role in mediating adaptive humoral immune defense.

This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of human IgA in the sample. Standards and samples are respectively added to the microplate wells pre-coated with an anti-human IgA antibody. Biotin-labeled IgA antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. The capture antibody pre-coated on the plate captures the IgA in the rat samples. IgA binds to the biotinylated anti-IgA human monoclonal antibody. And the biotin on the biotinylated anti-IgA human monoclonal antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of the TMB substrate. The addition of the stop solution into the wells immediately turns the blue into yellow. The concentration of IgA in the samples is directly proportional to OD (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.

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