Chicken Leptin,LEP ELISA kit

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Product Details

Description

This Chicken LEP ELISA Kit was designed for the quantitative measurement of Chicken LEP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Target Name

leptin

Alternative Names

LEP ELISA Kit; OB ELISA Kit; Leptin ELISA Kit; Obesity factor ELISA Kit

Abbreviation

LEP

Uniprot No.

O42164

Species

Gallus gallus (Chicken)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-101
1:2	Average %	95
	Range %	85-104
1:4	Average %	91
	Range %	87-100
1:8	Average %	93
	Range %	89-106

Recovery

The recovery of chicken LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	86-107
EDTA plasma (n=4)	95	88-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.540	2.472	2.506	2.373
10	1.879	1.899	1.889	1.756
5	1.138	1.123	1.131	0.998
2.5	0.731	0.717	0.724	0.591
1.25	0.411	0.402	0.407	0.274
0.625	0.308	0.315	0.312	0.179
0.312	0.200	0.209	0.205	0.072
0	0.131	0.135	0.133

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Background

Function (From Uniprot)	Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways.
Subcellular Location	Secreted.
Protein Families	Leptin family
Tissue Specificity	Not exclusively localized in adipose tissue but is also expressed in liver.

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Check Code：

Code	CSB-E14082C
Size	96T,5×96T,10×96T
Trial Size
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.