Chicken Leptin,LEP ELISA kit

Instructions
Code CSB-E14082C
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name leptin
Alternative Names LEP ELISA Kit; OB ELISA Kit; Leptin ELISA Kit; Obesity factor ELISA Kit
Abbreviation LEP
Uniprot No. O42164
Species Gallus gallus (Chicken)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %86-101
1:2Average %95
Range %85-104
1:4Average %91
Range %87-100
1:8Average %93
Range %89-106
Recovery
The recovery of chicken LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9786-107
EDTA plasma (n=4)9588-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.540 2.472 2.506 2.373
101.879 1.899 1.889 1.756
51.138 1.123 1.131 0.998
2.50.731 0.717 0.724 0.591
1.250.411 0.402 0.407 0.274
0.6250.308 0.315 0.312 0.179
0.3120.200 0.209 0.205 0.072
00.131 0.135 0.133
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways.
Subcellular Location Secreted
Protein Families Leptin family
Tissue Specificity Not exclusively localized in adipose tissue but is also expressed in liver.

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