Dog Complement 3(C3)ELISA Kit

Code CSB-EL003888DO
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Complement 3(C3)
Alternative Names
AHUS5, ARMD9, ASP, CPAMD1, acylation-stimulating protein cleavage product|complement component C3
Abbreviation
C3
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.78 μg/mL-50 μg/mL
Sensitivity
0.195 μg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 99  
Range % 95-102  
1:200 Average % 95  
Range % 81-89  
1:400 Average % 92  
Range % 88-96  
1:800 Average % 101  
Range % 98-103  
Recovery
The recovery of dog C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 91-97  
EDTA plasma (n=4) 89 85-93  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
50 0.075 0.079 0.077    
25 0.130 0.136 0.133    
12.5 0.206 0.204 0.205    
6.25 0.322 0.332 0.327    
3.12 0.543 0.538 0.541    
1.56 0.851 0.878 0.865    
0.78 1.163 1.198 1.181    
0 2.014 2.036 2.025    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Dog Complement 3 (C3) ELISA Kit was designed for the quantitative measurement of Dog Complement 3 (C3) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.78 μg/mL-50 μg/mL and the sensitivity is 0.195 μg/mL.

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