Rat Complement 3,C3 ELISA Kit

CloseMessage

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

CloseMessage

Product Details

Description

This Rat Complement 3 (C3) ELISA Kit was designed for the quantitative measurement of Rat Complement 3 (C3) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.625 μg/mL-40 μg/mL and the sensitivity is 0.156 μg/mL.

Target Name

Complement 3,C3

Alternative Names

C3Complement C3 [Cleaved into: Complement C3 beta chain; C3-beta-c ELISA kit; C3bc ELISA kit; Neutrophil chemotactic factor-2 ELISA kit; ENCF-2); Complement C3 alpha chain; C3a anaphylatoxin ELISA kit; Neutrophil chemotactic factor-1 ELISA kit; ENCF-1); Acylation stimulating protein ELISA kit; ASP ELISA kit; C3adesArg); Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3dg fragment; Complement C3g fragment; Complement C3d fragment; Complement C3f fragment; Complement C3c alpha' chain fragment 2] ELISA kit

Abbreviation

Uniprot No.

P01026

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.625 μg/mL-40 μg/mL

Sensitivity

0.156 μg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	84
	Range %	81-89
1:200	Average %	92
	Range %	87-96
1:400	Average %	101
	Range %	97-106
1:800	Average %	87
	Range %	84-90

Recovery

The recovery of rat C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	95	89-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average
40	0.085	0.083	0.084
20	0.173	0.182	0.178
10	0.308	0.321	0.315
5	0.576	0.608	0.592
2.5	0.983	0.961	0.972
1.25	1.462	1.465	1.464
0.625	1.825	1.862	1.844
0	2.553	2.567	2.560

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Related Products

Target Background

Function (From Uniprot)	C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.; Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. In chronic inflammation, acts as a chemoattractant for neutrophils.; Acts as a chemoattractant for neutrophils in chronic inflammation.; adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2.
Gene References into Functions	These data suggest that locally produced C3 is an important prosurvival mechanism in pancreatic beta-cells under a proinflammatory assault. PMID: 28582497 Inhibition of C3 might improve the reactivity of superior mesenteric arteries to norepinephrine during hemorrhagic shock possibly through the downregulation of NO, ET1, TNF-alpha and reactive oxygen radicals. PMID: 26687560 Recruited microglia/monocytes contribute to activation of complement in the aging retina, through local expression of C3 mRNA. PMID: 24705166 The C3 and C3a receptor system may primarily be involved in the pathogenesis of renal remodeling in hypertensive rats. PMID: 23713944 The expression of the complement component C3f and the complement component 4A have potential for use in post-transplant diagnosis of rejection. PMID: 23613499 Abnormal activation of complement C3 in the spinal dorsal horn is closely associated with progression of neuropathic pain. PMID: 23588254 The retinal irradiation with 670-nm light substantially reduces the propagation of complement3 in the retina following light-induced degeneration. PMID: 23181358 Early response to blast injury involves activation of complement and TNFalpha, correlating with hippocampus and cerebral cortex immune-inflammatory damage. PMID: 22537900 Carboxypeptidase B2 and complement C3 were more abundant in the cerebrospinal fluid of experimental autoimmune encephalomyelitis rats that did not respond to minocyline treatment. PMID: 22768796 Increase of C3 expression occurs in light damaged retina leading to retinal degeneration. PMID: 22183312 C3 and C4 may be involved in the immunopathology of allergic rhinitis. PMID: 17345707 Perivascular adipose tissue-derived C3 stimulated adventitial fibroblast migration and differentiation via the c-Jun N-terminal kinase pathway and may contribute to adventitial remodeling in a deoxycorticosterone acetate-salt hypertensive model. PMID: 20864665 downregulation in microglia while differentiating into ramified cells PMID: 12137932 complement C3 expression is regulated by the bile acid receptor FXR PMID: 15590640 Increased STAT-1, IRF-1 and complement component C3 in several models of liver transplant tolerance suggests that the STAT-1/IRF-1 apoptotic pathway and C3 may be involved in the tolerogenic mechanism. PMID: 16555329 Complement C1 Inactivator Proteins, in addition to inhibition of the systemic complement activation, prevents myocardial cell injury via a direct inhibitory role in the local myocardial C3 activity PMID: 16996480 C3a and C5a mediate distinct effects on blood pressure and circulating polymorphonuclear leukocytes in the rat. PMID: 19285573 role for C3 in influencing the level of expression of CR3 by modulating the transcript levels encoding the CD11b alpha integrin protein PMID: 19710459 Show More Hide All
Subcellular Location	Secreted.
Database Links	KEGG: rno:24232 STRING: 10116.ENSRNOP00000066885 UniGene: Rn.11378

Product >> Product Name：
Product >> Code：
Product >> Size：
Name： *
Email： *
Phone：
We recommend you to leave your phone number because occasionally our emails get flagged by spam filters. We would not disclose any of your private information to any third party.
Question/Comment： *
Your Region： *
Country State：
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
Check Code：

Code	CSB-E08666r
Size	96T,5×96T,10×96T How to order?
Trial Size
Have Questions?	Leave a Message or Start an on-line Chat

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.