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Dog Erythropoietin,EPO ELISA Kit

Protocol MSDS
Code CSB-E11303c
Size 96T,5×96T,10×96T
Price Request a Quote
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Product Details

Target Name
erythropoietin
Alternative Names
EPOErythropoietin ELISA kit
Abbreviation
EPO
Uniprot No.
P33707
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.56 mIU/mL-100 mIU/mL
Sensitivity
0.39 mIU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:1 Average % 97
Range % 91-105
1:2 Average % 89
Range % 80-97
1:4 Average % 93
Range % 86-100
1:8 Average % 95
Range % 88-100
Recovery
The recovery of dog EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 83-102
EDTA plasma (n=4) 95 85-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/ml OD1 OD2 Average Corrected
100 1.902 1.893 1.898 1.725
50 1.505 1.518 1.512 1.339
25 1.087 1.146 1.117 0.944
12.5 0.804 0.789 0.797 0.624
6.25 0.499 0.512 0.506 0.333
3.12 0.342 0.351 0.347 0.174
1.56 0.255 0.261 0.258 0.085
0 0.170 0.176 0.173 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Dog EPO ELISA Kit was designed for the quantitative measurement of Dog EPO protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 mIU/mL-100 mIU/mL and the sensitivity is 0.39 mIU/mL.

Related Products

EPO Proteins

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Target Background

Function
(From Uniprot)
Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
Gene References into Functions
  1. Developmental and compensatory lung growth involve paracrine erythropoietin signaling with parallel upregulation but differential processing of the erythropoietin receptor. PMID: 15286000
  2. apical secretion of EPO involves a cholesterol-dependent mechanism probably not involving lipid rafts PMID: 15916748
Subcellular Location
Secreted.
Protein Families
EPO/TPO family
Tissue Specificity
Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
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