Dog Interleukin-13(IL13) ELISA kit

Code CSB-EL011590DO
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
interleukin 13
Alternative Names
IL13 ELISA Kit; Interleukin-13 ELISA Kit; IL-13 ELISA Kit
Abbreviation
IL13
Uniprot No.
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
12.5 pg/mL-800 pg/mL
Sensitivity
3.12 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog IL13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 98
Range % 93-102
1:2 Average % 92
Range % 88-96
1:4 Average % 90
Range % 86-94
1:8 Average % 87
Range % 82-91
Recovery
The recovery of dog IL13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 104 100-112
EDTA plasma (n=4) 85 80-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
800 2.315 2.467 2.391 2.263
400 2.049 2.158 2.104 1.976
200 1.720 1.680 1.700 1.572
100 1.193 1.124 1.159 1.031
50 0.782 0.796 0.789 0.661
25 0.507 0.475 0.491 0.363
12.5 0.328 0.309 0.319 0.191
0 0.124 0.131 0.128  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Dog IL13 ELISA Kit was designed for the quantitative measurement of Dog IL13 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.

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Target Background

Function
(From Uniprot)
Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages.
Subcellular Location
Secreted.
Protein Families
IL-4/IL-13 family
Database Links
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