Dog Interleukin-13(IL13) ELISA kit

Code CSB-EL011590DO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 13
Alternative Names IL13 ELISA Kit; Interleukin-13 ELISA Kit; IL-13 ELISA Kit
Abbreviation IL13
Uniprot No. Q9N0W9
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, tissue homogenates
Detection Range 12.5 pg/mL-800 pg/mL
Sensitivity 3.12 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog IL13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 98
Range % 93-102
1:2 Average % 92
Range % 88-96
1:4 Average % 90
Range % 86-94
1:8 Average % 87
Range % 82-91
Recovery
The recovery of dog IL13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 104 100-112
EDTA plasma (n=4) 85 80-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
800 2.315 2.467 2.391 2.263
400 2.049 2.158 2.104 1.976
200 1.720 1.680 1.700 1.572
100 1.193 1.124 1.159 1.031
50 0.782 0.796 0.789 0.661
25 0.507 0.475 0.491 0.363
12.5 0.328 0.309 0.319 0.191
0 0.124 0.131 0.128  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages.
Subcellular Location Secreted.
Protein Families IL-4/IL-13 family
Database Links

KEGG: cfa:442990

STRING: 9615.ENSCAFP00000001250

UniGene: Cfa.3566

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