Dog Matrix metalloproteinase 3,MMP-3 ELISA kit

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Dog Matrix metalloproteinase 3,MMP-3 ELISA kit

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Code	CSB-E13621c
Size	96T,5×96T,10×96T
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Citations (2)
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Description

This Dog MMP3 ELISA Kit was designed for the quantitative measurement of Dog MMP3 protein in serum, plasma, cell culture supernates, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL .

Target Name

matrix metallopeptidase 3 (stromelysin 1, progelatinase)

Alternative Names

MMP3Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit

Abbreviation

MMP3

Uniprot No.

Q6Y4Q5

Species

Canis lupus familiaris (Dog) (Canis familiaris)

Sample Types

serum, plasma, cell culture supernates, urine, tissue homogenates

Detection Range

1.56 ng/mL-100 ng/mL

Sensitivity

0.39 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	89
	Range %	85-96
1:4	Average %	93
	Range %	89-97
1:8	Average %	99
	Range %	92-105

Recovery

The recovery of dog MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	92	87-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.410	2.545	2.478	2.284
50	1.960	1.982	1.971	1.777
25	1.595	1.563	1.579	1.385
12.5	1.177	1.201	1.189	0.995
6.25	0.786	0.789	0.788	0.594
3.12	0.458	0.462	0.460	0.266
1.56	0.278	0.262	0.270	0.076
0	0.193	0.195	0.194

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Inflammatory pattern of the infrapatellar fat pad in dogs with canine cruciate ligament disease Manuel R. Schmidli.et al,BMC VET RES,2018
Evaluation of Glucosamine and Snail Mucin on the Progression of Experimental Knee Osteoarthritis in Dogs Adetola R. Ajadi et al,International Journal of Morphology,2013

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Function (From Uniprot)	Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
Gene References into Functions	Findings suggested that increased TGF-beta3 and MMP-3 contribute to the pathogenesis of valvular degeneration associated with chronic mitral valve disease. PMID: 21281193 Both mRNA transcript levels and the activities of MMP-3 and MMP-7 are elevated in X-linked Alport syndrome (XLAS) kidneys from dogs. PMID: 15782307
Subcellular Location	Secreted, extracellular space, extracellular matrix.
Protein Families	Peptidase M10A family
Database Links	KEGG: cfa:403445 STRING: 9615.ENSCAFP00000022195 UniGene: Cfa.3447

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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