Recombinant Dog Stromelysin-1 (MMP3)

Code CSB-YP761568DO
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Source Yeast
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Code CSB-EP761568DO
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Source E.coli
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Code CSB-EP761568DO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP761568DO
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Source Baculovirus
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Code CSB-MP761568DO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MMP3
Uniprot No.
Alternative Names
MMP3Stromelysin-1; SL-1; EC 3.4.24.17; Matrix metalloproteinase-3; MMP-3
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Expression Region
100-478
Target Protein Sequence
F TTFPGMPKWR KTHLTYRIMN YTPDLPRDAV DSAIEKALNV WKEVTPLTFS RTDEGEADIK ISFAVRDHGD FNPFDGPGNV LGHAYPPGPG IYGDAHFDDD EQWTSDTSGT NLFLVAAHEL GHSLGLFHSA DPSALMYPVY NVLADLARFH LSQDDVNGIQ SLYGGPPSDS SNDPVVPTES VPPGPGTPAA CDPTLSFDAI STLRGEFLFF KDRHFWRKSL RTLEPGFYLI SSFWPSLPSG LDAAYEETSK DIVFIFKGNQ FWAMRGTEVQ AGYPKGIHTL GFPPTVKKID AAVFDKEKKK TYFFVGDKYW RFDEKRQSME PGFPKQIAED FPGVDSKVDA AFEAFGFYYF FNGSSQLEFD PNAKKVTHVL KSNSWLNC
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
Gene References into Functions
  1. Findings suggested that increased TGF-beta3 and MMP-3 contribute to the pathogenesis of valvular degeneration associated with chronic mitral valve disease. PMID: 21281193
  2. Both mRNA transcript levels and the activities of MMP-3 and MMP-7 are elevated in X-linked Alport syndrome (XLAS) kidneys from dogs. PMID: 15782307
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Peptidase M10A family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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