Dog Renin ELISA Kit

Code CSB-E08704c
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
renin
Alternative Names
REN ELISA Kit; Renin ELISA Kit; EC 3.4.23.15 ELISA Kit; Angiotensinogenase ELISA Kit
Abbreviation
REN
Uniprot No.
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
62.5 pg/mL-4000 pg/mL
Sensitivity
15.6 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog renin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %89-97
1:2Average %105
Range %100-112
1:4Average %84
Range %80-88
1:8Average %95
Range %90-100
Recovery
The recovery of dog renin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9790-103
EDTA plasma (n=4)8884-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1OD2AverageCorrected
40002.582 2.683 2.633 2.454
20002.204 2.104 2.154 1.975
10001.451 1.521 1.486 1.307
5000.864 0.908 0.886 0.707
2500.569 0.584 0.577 0.398
1250.430 0.402 0.416 0.237
62.50.279 0.259 0.269 0.090
00.182 0.176 0.179
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Dog REN ELISA Kit was designed for the quantitative measurement of Dog REN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

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Target Background

Function
(From Uniprot)
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Gene References into Functions
  1. With a moderate deficit in total body sodium and total body water, the renin-angiotensin-aldosterone system is the major factor controlling arterial blood pressure. PMID: 19930429
  2. Characterization of canine renin with mutations in the substrate binding site. PMID: 17942478
  3. Normotensive sodium loading in conscious dogs: regulation of renin secretion during beta1-receptor blockade. PMID: 19073902
Subcellular Location
Secreted. Membrane.
Protein Families
Peptidase A1 family
Database Links
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