Dog adrenomedullin,ADM ELISA Kit

Code CSB-E09836c
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Dog ADM ELISA Kit was designed for the quantitative measurement of Dog ADM protein in serum, plasma, tissue homogenates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

Target Name adrenomedullin
Alternative Names ADM ELISA Kit; ADM [Cleaved into: Adrenomedullin ELISA Kit; AM); Proadrenomedullin N-20 terminal peptide ELISA Kit; ProAM N-terminal 20 peptide ELISA Kit; PAMP ELISA Kit; ProAM-N20)] ELISA Kit
Abbreviation ADM
Uniprot No. O77559
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, tissue homogenates, cell culture supernates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog ADM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %80-99
1:2Average %96
Range %91-104
1:4Average %98
Range %90-110
1:8Average %95
Range %85-105
Recovery
The recovery of dog ADM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9582-104
EDTA plasma (n=4)9790-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.675 2.641 2.658 2.502
5002.159 2.144 2.152 1.996
2501.312 1.310 1.311 1.155
1250.778 0.788 0.783 0.627
62.50.377 0.360 0.369 0.213
31.250.276 0.264 0.270 0.114
15.60.190 0.195 0.193 0.037
00.154 0.158 0.156  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
AM and PAMP are potent hypotensive and vasodilatator agents.
Subcellular Location Secreted.
Protein Families Adrenomedullin family
Database Links

KEGG: cfa:403817

STRING: 9615.ENSCAFP00000011183

UniGene: Cfa.3681

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Address
7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
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