Dog interleukin 4,IL-4 ELISA Kit

Instructions
Code CSB-E11259c
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 4
Alternative Names IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
Abbreviation IL4
Uniprot No. O77762
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 87-97  
1:2 Average % 89  
Range % 85-99  
1:4 Average % 98  
Range % 92-105  
1:8 Average % 93  
Range % 86-100  
Recovery
The recovery of dog IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-105  
EDTA plasma (n=4) 92 85-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
400 2.912 2.986 2.949 2.853  
200 2.546 2.531 2.539 2.443  
100 1.780 1.831 1.806 1.710  
50 1.127 1.207 1.167 1.071  
25 0.546 0.588 0.567 0.471  
12.5 0.259 0.276 0.268 0.172  
6.25 0.168 0.176 0.172 0.076  
0 0.095 0.096 0.096    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
Gene References into Functions
  1. immunoreactivities of inflammatory cytokines, such as interleukin (IL)-2, its receptor (IL-2R), IL-4 and its receptor (IL-4R) in the cervical and lumbar spinal cord of young adult and aged beagle dogs, were compared. PMID: 23605681
  2. In dogs with leishmaniasis, there was high expression of genes encoding IL-4 in blood leucocytes. The predominance of IL-4 gene expression in the blood of asymptomatic dogs may favour parasite replication. PMID: 21511273
  3. The anti-inflammatory and anabolic effects of regulated expression of IL-4 in chondrocyte-scaffolds under in vitro inflammatory conditions, were investigated. PMID: 21991344
  4. This study focuses on the co-expression of interleukin-4 (IL-4) and insulin-like-growth factor-1 (IGF-1), which specifically target inflammation and cartilage repair, respectively. PMID: 20188584
  5. These findings suggest that polyunsaturated fatty acids are able to influence proliferation of peripheral blood mononuclear cells in healthy and atopic dogs but do not seem to influence cytokine transcription. PMID: 20187917
  6. IL-4 expressed in canine articular chondrocytes is biologically active and suppresses inflammatory mediators in vitro. PMID: 18789870

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Subcellular Location Secreted.
Protein Families IL-4/IL-13 family
Database Links

KEGG: cfa:403785

STRING: 9615.ENSCAFP00000001253

UniGene: Cfa.39

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