Fish Heat Shock Protein 70(HSP-70)ELISA Kit

Code CSB-E16327Fh
Size 96T,5×96T,10×96T
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Product Details

Target Name
Heat Shock Protein 70(HSP-70)
Alternative Names
Heat shock 70 kDa protein 1 ELISA Kit; HSP70-1 ELISA Kit
Abbreviation
HSP70
Uniprot No.
Species
Fish
Sample Types
serum, plasma, tissue homogenates
Detection Range
18.75 pg/mL-300 pg/mL
Sensitivity
18.75 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 86-95  
1:2 Average % 98  
Range % 92-103  
1:4 Average % 82  
Range % 80-85  
1:8 Average % 91  
Range % 83-99  
Recovery
The recovery of fish HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-97  
EDTA plasma (n=4) 94 91-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
300 0.153 0.144 0.149    
150 0.255 0.242 0.249    
75 0.519 0.533 0.526    
37.5 1.103 1.134 1.119    
18.75 2.363 2.270 2.317    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

Heat Shock Protein 70 (HSP-70) is a highly conserved molecular chaperone that plays a key role in protein folding, cellular stress response, and cytoprotection across diverse species. This protein is rapidly upregulated in response to various stressors including elevated temperature, oxidative stress, and environmental toxins. HSP-70 serves as a valuable biomarker for cellular stress and adaptive responses. In aquatic organisms, HSP-70 expression acts as an important indicator of environmental stress and has been widely studied in ecotoxicology and fish health research.

The Fish Heat Shock Protein 70 ELISA Kit (CSB-E16327Fh) is a quantitative competitive assay designed for measuring HSP-70 levels in fish samples. This kit accommodates serum, plasma, and tissue homogenates with a detection range of 18.75 pg/mL to 300 pg/mL and a sensitivity of 18.75 pg/mL. The assay requires 50-100 μL sample volume, operates at 450 nm detection wavelength, and can be completed within 1-5 hours.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in aquaculture research to measure heat shock protein levels in fish plasma as part of comprehensive stress and immune response studies. The kit provides a valuable tool for evaluating physiological responses in fish subjected to various experimental conditions and environmental stressors.

Stress response studies: Measurement of heat shock protein levels in fish plasma to evaluate cellular stress responses alongside other stress indicators and immune parameters
Aquaculture research: Monitoring of physiological biomarkers in fish to understand responses to experimental treatments and environmental conditions
Multi-parameter analysis: Integration with broader biomarker panels to provide comprehensive profiles of fish health and stress status in controlled research settings

Citations

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