Code | CSB-E16327Fh |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
|
Have Questions? | Leave a Message or Start an on-line Chat |
Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of fish HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 91 | ||||
Range % | 86-95 | |||||
1:2 | Average % | 98 | ||||
Range % | 92-103 | |||||
1:4 | Average % | 82 | ||||
Range % | 80-85 | |||||
1:8 | Average % | 91 | ||||
Range % | 83-99 |
The recovery of fish HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 92 | 87-97 | ||||
EDTA plasma (n=4) | 94 | 91-98 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||
![]()
|
Heat Shock Protein 70 (HSP-70) is a highly conserved molecular chaperone that plays a key role in protein folding, cellular stress response, and cytoprotection across diverse species. This protein is rapidly upregulated in response to various stressors including elevated temperature, oxidative stress, and environmental toxins. HSP-70 serves as a valuable biomarker for cellular stress and adaptive responses. In aquatic organisms, HSP-70 expression acts as an important indicator of environmental stress and has been widely studied in ecotoxicology and fish health research.
The Fish Heat Shock Protein 70 ELISA Kit (CSB-E16327Fh) is a quantitative competitive assay designed for measuring HSP-70 levels in fish samples. This kit accommodates serum, plasma, and tissue homogenates with a detection range of 18.75 pg/mL to 300 pg/mL and a sensitivity of 18.75 pg/mL. The assay requires 50-100 μL sample volume, operates at 450 nm detection wavelength, and can be completed within 1-5 hours.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This ELISA kit has been used in aquaculture research to measure heat shock protein levels in fish plasma as part of comprehensive stress and immune response studies. The kit provides a valuable tool for evaluating physiological responses in fish subjected to various experimental conditions and environmental stressors.
• Stress response studies: Measurement of heat shock protein levels in fish plasma to evaluate cellular stress responses alongside other stress indicators and immune parameters
• Aquaculture research: Monitoring of physiological biomarkers in fish to understand responses to experimental treatments and environmental conditions
• Multi-parameter analysis: Integration with broader biomarker panels to provide comprehensive profiles of fish health and stress status in controlled research settings
There are currently no reviews for this product.