Fish Heat Shock Protein 70(HSP-70)ELISA Kit

Fish Heat Shock Protein 70(HSP-70)ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

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Customer Reviews and Q&A

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Fish Heat Shock Protein 70(HSP-70)ELISA Kit

Citations (10) Protocol

Code	CSB-E16327Fh
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details
Citations (10)
Related Products
Customer Reviews and Q&A

Target Name

Heat Shock Protein 70(HSP-70)

Alternative Names

Heat shock 70 kDa protein 1 ELISA Kit; HSP70-1 ELISA Kit

Abbreviation

HSP70

Uniprot No.

Q9I8F9

Species

Fish

Sample Types

serum, plasma, tissue homogenates

Detection Range

18.75 pg/mL-300 pg/mL

Sensitivity

18.75 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	98
	Range %	92-103
1:4	Average %	82
	Range %	80-85
1:8	Average %	91
	Range %	83-99

Recovery

The recovery of fish HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	94	91-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
300	0.153	0.144	0.149
150	0.255	0.242	0.249
75	0.519	0.533	0.526
37.5	1.103	1.134	1.119
18.75	2.363	2.270	2.317

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Fish HSP70 ELISA Kit was designed for the quantitative measurement of Fish HSP70 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 18.75 pg/mL-300 pg/mL and the sensitivity is 18.75 pg/mL.

Growth, Nutrient Deposition, Plasma Metabolites, and Innate Immunity are Associated with Feeding Rate in Juvenile Starry Flounder (Platichthys Stellatus) JH Cho,/,/
TNF-Α, IL-6, HSP-70, fish growth hormone, and growth performance of sea trout, Salmo trutta (Actinopterygii: Salmoniformes: Salmonidae) after long-term dietary administration of β-glucan and BGN-2 O Revina,/,2023
Cadmium induced oxidative stress, endoplasmic reticulum (ER) stress and apoptosis with compensative responses towards the up-regulation of ribosome, protein processing in the ER, and protein export pathways in the liver of zebrafish W Hu,Aquatic toxicology,2023
Effects of black soldier fly (Hermetia illucens) pre-pupae meal on the growth, stress, and immune responses of juvenile rainbow trout (Oncorhynchus mykiss) reared at different stocking densities JH Cho,Aquaculture Reports,2022
Effect of temperature on food consumption, immune system, antioxidant enzymes, and heat shock protein 70 of Channa punctata (Bloch, 1793). SP Singh,fish physiology and biochemistry,2020
Effect of Light Intensity on Survival, Growth and Physiology of Rohu, Labeo rohita (Cyprinidae) Fry Khan N A, et al,International Journal of Radiation Biology,2019
INVESTIGATING THE ROLE OF HEAT SHOCK PROTEINS AND MELANOPHORES DURING STRESS CAUSED BY HIGH LIGHT INTENSITY IN INDIAN MAJOR CARP, CATLA CATLA N. A. Khan, et al,International Journal of Pharmaceutical Sciences and Research,2018
Dietary supplementation of vitamin C: an effective measure to give protection against UV-B irradiation using fish as model organism Rina Chakrabarti, et al,Photochemical & Photobiological Sciences,2018
Therapeutic effects of C-28 methyl ester of 2-cyano-3,12-dioxoolean-1,9-dien-28-oic acid (CDDO-Me; bardoxolone methyl) on radiation-induced lung inflammation and fibrosis in mice Wang YY. et al,Drug Des Devel Ther,2015
Vessel noise pollution as a human threat to fish: assessment of the stress response in gilthead sea bream (Sparus aurata, Linnaeus 1758) Celi M. et al,Fish Physiol Biochem,2015

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.