Goat growth hormone,GH ELISA KIT

Instructions
Code CSB-E13275G
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name growth hormone 1
Alternative Names GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
Abbreviation GH1
Uniprot No. P67931
Species Capra hircus (Goat)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 ng/mL-100 ng/mL
Sensitivity 6.25 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of goat GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-88  
1:2 Average % 87  
Range % 84-90  
1:4 Average % 95  
Range % 91-99  
1:8 Average % 92  
Range % 88-97  
Recovery
The recovery of goat GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-99  
EDTA plasma (n=4) 94 89-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
100 0.202 0.200 0.201    
50 0.396 0.365 0.381    
25 0.631 0.622 0.627    
12.5 1.142 1.154 1.148    
6.25 2.359 2.326 2.343    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Q&A and Customer Reviews

 Q&A
Q:

I'm studying metabolic stress in dairy goats in the peripartum.
In total, 450 samples have to be analysed for each hormone, and analysis will be done in duplicate. Meaning I'll need a number of kits enough to run 900 tests.
Could you please send me a quote specifying the plate size (i.e. 96 well) and the number of free wells per plate. (i.e. 96 well minus the number of wells used for the standard curve).

A:
Thanks for your inquiry. We have the mentioned kit available.
For example:#CSB-E13275G,Goat growth hormone,GH ELISA KIT *96T (If duplicate done,22 kits is advised;If not,only 11 kits is ok)
The standard wells are S1-S5 and duplicate is advised.With a blank well,the number of standard wells is 5×2+1=11.The rest is for samples wells,85=96-11(If duplicate is done for sample,then the number will be the half of that.
If you want to detect diary goat samples,we suggest you do pre-testing first.

Target Data

Function Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
Gene References into Functions
  1. genetic variation and milk production and composition PMID: 23611522
Subcellular Location Secreted
Protein Families Somatotropin/prolactin family
Database Links

KEGG: chx:100861230

UniGene: Chi.38639

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