Hamster Putative phospholipase B-like 2(PLBD2) ELISA kit

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Hamster Putative phospholipase B-like 2(PLBD2) ELISA kit

Citations (2) Protocol MSDS

Code	CSB-EL018125HA
Size	96T,5×96T,10×96T
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Product Details
Citations (2)
Customer Reviews and Q&A

Target Name

phospholipase B domain containing 2

Alternative Names

Abbreviation

PLBD2

Species

Mesocricetus auratus (Golden hamster)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

12.5 pg/mL-800 pg/mL

Sensitivity

3.12 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of hamster PLBD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-105
1:2	Average %	92
	Range %	86-96
1:4	Average %	95
	Range %	90-100
1:8	Average %	95
	Range %	91-100

Recovery

The recovery of hamster PLBD2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	96	89-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.349	2.367	2.358	2.227
400	1.975	1.924	1.950	1.819
200	1.408	1.466	1.437	1.306
100	0.912	0.857	0.885	0.754
50	0.555	0.521	0.538	0.407
25	0.363	0.374	0.369	0.238
12.5	0.248	0.259	0.254	0.123
0	0.133	0.129	0.131

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Hamster PLBD2 ELISA Kit was designed for the quantitative measurement of Hamster PLBD2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL .

Method of Characterization of Visible and/or Sub-Visible Particles in Biologics X Xu,freepatentsonline,2020
Compositions and methods for detecting and quantifying host cell protein in cell lines and recombinant polypeptide products Feny Gunawan, et al,/,2018

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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