Hamster Putative phospholipase B-like 2(PLBD2) ELISA kit

Instructions
Code CSB-EL018125HA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name phospholipase B domain containing 2
Alternative Names P76, LAMA-like protein 2|PLB homolog 2|lamina ancestor homolog 2|mannose-6-phosphate protein associated protein p76|phospholipase B-like 2 32 kDa form|phospholipase B-like 2 45 kDa form
Abbreviation PLBD2
Species Mesocricetus auratus (Golden hamster)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 12.5 pg/mL-800 pg/mL
Sensitivity 3.12 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of hamster PLBD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 90-105  
1:2 Average % 92  
Range % 86-96  
1:4 Average % 95  
Range % 90-100  
1:8 Average % 95  
Range % 91-100  
Recovery
The recovery of hamster PLBD2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 87-94  
EDTA plasma (n=4) 96 89-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
800 2.349 2.367 2.358 2.227  
400 1.975 1.924 1.950 1.819  
200 1.408 1.466 1.437 1.306  
100 0.912 0.857 0.885 0.754  
50 0.555 0.521 0.538 0.407  
25 0.363 0.374 0.369 0.238  
12.5 0.248 0.259 0.254 0.123  
0 0.133 0.129 0.131    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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