Hamster adiponectin, C1Q and collagen domain containing (ADIPOQ)ELISA kit

Instructions
Code CSB-EL001366HA
Size 96T,5×96T,10×96T
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Product Details

Target Name adiponectin, C1Q and collagen domain containing (ADIPOQ)
Abbreviation ADIPOQ
Uniprot No. C9DFG2
Species Mesocricetus auratus (Golden hamster)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.469 ng/mL-30 ng/mL
Sensitivity 0.117 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of hamster ADIPOQ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1000Average %85
Range %82-90
1:2000Average %93
Range %90-96
1:4000Average %89
Range %84-93
1:8000Average %94
Range %90-98
Recovery
The recovery of hamster ADIPOQ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8884-92
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
302.703 2.768 2.736 2.595
152.244 2.201 2.223 2.082
7.51.688 1.698 1.693 1.552
3.751.073 1.054 1.064 0.923
1.8750.611 0.635 0.623 0.482
0.9380.321 0.342 0.332 0.191
0.4690.215 0.230 0.223 0.082
00.140 0.142 0.141
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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