Hamster adiponectin, C1Q and collagen domain containing (ADIPOQ)ELISA kit

Code CSB-EL001366HA
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. C9DFG2
Lead Time 7-14 working days
Abbreviation ADIPOQ
Protein Biological Process 1 Metabolism
Target Name adiponectin, C1Q and collagen domain containing (ADIPOQ)
Species Mesocricetus auratus (Golden hamster)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.469 ng/mL-30 ng/mL
Sensitivity 0.117 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of hamster ADIPOQ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1000Average %85
Range %82-90
1:2000Average %93
Range %90-96
1:4000Average %89
Range %84-93
1:8000Average %94
Range %90-98
Recovery
The recovery of hamster ADIPOQ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8884-92
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
302.703 2.768 2.736 2.595
152.244 2.201 2.223 2.082
7.51.688 1.698 1.693 1.552
3.751.073 1.054 1.064 0.923
1.8750.611 0.635 0.623 0.482
0.9380.321 0.342 0.332 0.191
0.4690.215 0.230 0.223 0.082
00.140 0.142 0.141
Still Have Questions? Leave a Message or Start an on-line Chat

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.

Wait!

Join the 25,000 subscribers to get research hotpots, technical tips, latest information on events, sales and offers.

Sign up now!

We don't deal in spam.