Hamster adiponectin, C1Q and collagen domain containing (ADIPOQ)ELISA kit

Code CSB-EL001366HA
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. C9DFG2
Lead Time 7-14 working days
Abbreviation ADIPOQ
Protein Biological Process 1 Metabolism
Target Name adiponectin, C1Q and collagen domain containing (ADIPOQ)
Species Mesocricetus auratus (Golden hamster)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.469 ng/mL-30 ng/mL
Sensitivity 0.117 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes.
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of hamster ADIPOQ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1000Average %85
Range %82-90
1:2000Average %93
Range %90-96
1:4000Average %89
Range %84-93
1:8000Average %94
Range %90-98
The recovery of hamster ADIPOQ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8884-92
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
302.703 2.768 2.736 2.595
152.244 2.201 2.223 2.082
7.51.688 1.698 1.693 1.552
3.751.073 1.054 1.064 0.923
1.8750.611 0.635 0.623 0.482
0.9380.321 0.342 0.332 0.191
0.4690.215 0.230 0.223 0.082
00.140 0.142 0.141
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