Human β-defensins 2 ELISA Kit

Code CSB-E13201h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human DEFB4 ELISA Kit was designed for the quantitative measurement of Human DEFB4 protein in serum, urine, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

Target Name defensin, beta 4
Alternative Names BD 2 ELISA Kit; BD-2 ELISA Kit; BD2 ELISA Kit; beta 2 ELISA Kit; beta 2 Defensin ELISA Kit; Beta defensin 2 ELISA Kit; Beta defensin 4B ELISA Kit; Beta-defensin 2 ELISA Kit; Beta-defensin 4A ELISA Kit; DEF B2 ELISA Kit; DEF B4 ELISA Kit; DEFB 102 ELISA Kit; DEFB 2 ELISA Kit; DEFB 4 ELISA Kit; DEFB102 ELISA Kit; DEFB2 ELISA Kit; DEFB2; formerly ELISA Kit; DEFB4 ELISA Kit; DEFB4; formerly ELISA Kit; DEFB4B ELISA Kit; DEFB4P ELISA Kit; Defensin ELISA Kit; Defensin beta 2 ELISA Kit; Defensin; beta 4 ELISA Kit; Defensin; beta 4; pseudogene ELISA Kit; Defensin; beta 4A ELISA Kit; Defensin; beta 4B ELISA Kit; Defensin; beta; 2; formerly ELISA Kit; Defensin; beta; 4; formerly ELISA Kit; DFB4A_HUMAN ELISA Kit; HBD 2 ELISA Kit; hBD-2 ELISA Kit; HBD2 ELISA Kit; mBD-2 ELISA Kit; SAP 1 ELISA Kit; SAP1 ELISA Kit; Skin antimicrobial peptide 1 ELISA Kit; Skin-antimicrobial peptide 1 ELISA Kit
Abbreviation DEFB4
Uniprot No. O15263
Species Homo sapiens (Human)
Sample Types serum, urine, cell culture supernates, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Microbiology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human β-defensins 2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %81-92
1:2Average %94
Range %90-100
1:4Average %98
Range %91-110
1:8Average %91
Range %85-98
Recovery
The recovery of human β-defensins 2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9389-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.821 2.752 2.787 2.689
20002.182 2.076 2.129 2.031
10001.438 1.364 1.401 1.303
5000.802 0.793 0.798 0.700
2500.471 0.437 0.454 0.356
1250.302 0.290 0.296 0.198
62.50.243 0.229 0.236 0.138
00.099 0.097 0.098  
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human DEFB4 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled DEFB4 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria. May act as a ligand for C-C chemokine receptor CCR6. Can bind to both human and mouse CCR6 and induce chemotactic activity of CCR6-expressing cells.
Gene References into Functions
  1. The encoded peptide has antimicrobial activity against E.coli, S. aureus, and P. aeruginosa. PMID: 9727055
Subcellular Location Secreted.
Protein Families Beta-defensin family, LAP/TAP subfamily
Tissue Specificity Expressed in the skin and respiratory tract.
Database Links

HGNC: 2767

OMIM: 602215

KEGG: hsa:100289462

STRING: 9606.ENSP00000424598

UniGene: Hs.105924

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