Human 4-Hydroxynonenal (HNE)ELISA Kit

Human 4-Hydroxynonenal (HNE)ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

Related Products

Customer Reviews and Q&A

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Human 4-Hydroxynonenal (HNE)ELISA Kit

Citations (18) Protocol

Code	CSB-E16214h
Size	96T,5×96T,10×96T
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Product Details
Citations (18)
Related Products
Customer Reviews and Q&A

Target Name

4-Hydroxynonenal (HNE)

Abbreviation

HNE

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

39 pg/mL-2500 pg/mL

Sensitivity

19.5 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human HNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:100	Average %	89
	Range %	82-96
1:200	Average %	90
	Range %	85-96
1:400	Average %	85
	Range %	81-92
1:800	Average %	98
	Range %	91-104

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

2500

0.175

0.170

0.173

1250

0.261

0.273

0.267

625

0.383

0.408

0.396

312.5

0.669

0.629

0.649

156.25

1.008

1.021

1.014

1.345

1.353

1.349

1.879

1.784

1.832

2.611

2.525

2.568

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human HNE ELISA Kit was designed for the quantitative measurement of Human HNE protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 39 pg/mL-2500 pg/mL and the sensitivity is 19.5 pg/mL.

Insulin Resistance and 4-Hydroxynonenal in Obesity-Associated Lipid Peroxidation of Adipocytes: A Comparative Cross-Sectional Study S Ramsridhar, C Rajkumar, PN Adtani,Journal of International Dental and Medical Research,2024
Treatment of human sperm with GYY4137 increases sperm motility and resistance to oxidative stress Y Huang, R Gan, M Zhang, D Lin, Y Cheng, X Guo,Zygote,2024
A Randomised, Double-Blind, Placebo-Controlled, Cross-Over Clinical Trial to Evaluate the Biological Effects and Safety of a Polyphenol Supplement on Healthy Ageing JR Chong, C de Lucia, DA Tovar-Rios,Antioxidants,2024
COPZ1 regulates ferroptosis through NCOA4-mediated ferritinophagy in lung adenocarcinoma A Wu,/,2023
Genome-Wide Screening Identifies Gene AKR1C1 Critical for Resistance to Pirarubicin in Bladder Cancer Z Nie,Cancers,2023
The Influence of Winter Swimming on Oxidative Stress Indicators in the Blood of Healthy Males R Wesołowski,Metabolites,2023
Serum Level of 4-Hydroxynonenal in Community-Acquired Pneumonia: A Potential Biomarker for Severity and Prognosis L Fu,Frontiers in Medicine,2022
Do different lipid components accelerate the pathogenesis and severity of Diabetic Retinopathy? Lakshmi Kanta Mondal,International Journal of Retina and Vitreous,2022
Sunitinib and Pterostilbene Combination Treatment Exerts Antitumor Effects in Gastric Cancer via Suppression of PDZD8 Y Hojo,International journal of molecular sciences,2022
Role of Lipid, Protein-Derived Toxic Molecules, and Deficiency of Antioxidants behind the Pathogenesis of Diabetic Retinopathy (DR) in Type 2 Diabetes Mellitus S Pramanik,/,2021
Oxidative stress in lung cancer patients is associated with altered serum markers of lipid metabolism Zablocka-Slowinska K, et al,PLoS One,2019
Melatonin supplementation lowers oxidative stress and regulates adipokines in obese patients on a calorie-restricted diet Karolina Szewczykolec .et al,/,/
4-hydroxynonenal causes impairment of human subcutaneous adipogenesis and induction of adipocyte insulin resistance Mohamed Elrayess.et al,Free Radical Biology and Medicine,2017
Melatonin Supplementation Lowers Oxidative Stress and Regulates Adipokines in Obese Patients on a Calorie-Restricted Diet Karolina Szewczyk-Golec.et al,Oxidative Medicine and Cellular Longevity,2017
4-Hydroxynonenal Regulates TNF-a Gene Transcription Indirectly via ETS1 and microRNA-29b in Human Adipocytes Induced From Adipose Tissue-Derived Stromal Cells. Zhang XM.et al,Anat Rec (Hoboken).,2016
Asociación del hydroxynonenal 4 con las adipoquinas clásicas y la resistencia a la insulina en una población china obesa no diabética Guo, Lin.et al,Nutricin Hospitalaria,2016
Oxidized Docosahexaenoic Acid Species and Lipid Peroxidation Products Increase Amyloidogenic Amyloid Precursor Protein Processing Grimm MO. et al,Neurodegener Dis,2015
Naturally occurring variation in the glutathione-S-transferase 4 gene determines neurodegeneration after traumatic brain injury /,Antioxidants & Redox Signaling,2013

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.