Mouse 4-Hydroxynonenal (HNE)ELISA Kit

Code CSB-E13412m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name 4-Hydroxynonenal (HNE)
Abbreviation HNE
Species Mus musculus (Mouse)
Sample Types serum, plasma
Detection Range 15.6 pmol/mL-1000 pmol/mL
Sensitivity 3.9 pmol/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %92
Range %87-98
1:2Average %96
Range %91-103
1:4Average %94
Range %90-99
1:8Average %94
Range %88-98
The recovery of mouse HNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9289-97
EDTA plasma (n=4)9490-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
10002.608 2.555 2.582 2.392
5001.939 1.897 1.918 1.728
2501.227 1.212 1.220 1.030
1250.821 0.759 0.790 0.600
62.50.468 0.450 0.459 0.269
31.20.331 0.328 0.330 0.140
15.60.278 0.271 0.275 0.085
00.192 0.188 0.190  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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