Rat 4-Hydroxynonenal(HNE)ELISA Kit

Instructions
Code CSB-EQ027232RA
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name 4-Hydroxynonenal(HNE)
Abbreviation HNE
Species Rattus norvegicus (Rat)
Sample Types serum, plasma
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat HNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 87  
Range % 83-92  
1:200 Average % 96  
Range % 89-99  
1:400 Average % 95  
Range % 91-100  
1:800 Average % 93  
Range % 87-98  
Recovery
The recovery of rat HNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 89-95  
EDTA plasma (n=4) 98 94-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
1000 0.118 0.110 0.114    
500 0.184 0.192 0.188    
250 0.265 0.273 0.269    
125 0.474 0.466 0.470    
62.5 0.625 0.615 0.620    
31.2 1.129 1.074 1.102    
15.6 2.182 2.281 2.232    
0 2.877 2.985 2.931    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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