Rat 4-Hydroxynonenal(HNE)ELISA Kit

Rat 4-Hydroxynonenal(HNE)ELISA Kit

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Citations

Customer Reviews and Q&A

Product Details

Citations

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Customer Reviews and Q&A

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Rat 4-Hydroxynonenal(HNE)ELISA Kit

Citations (7) Protocol

Code	CSB-EQ027232RA
Size	96T,5×96T,10×96T
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Product Details
Citations (7)
Related Products
Customer Reviews and Q&A

Target Name

4-Hydroxynonenal(HNE)

Abbreviation

HNE

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

7.8 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat HNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	87
	Range %	83-92
1:200	Average %	96
	Range %	89-99
1:400	Average %	95
	Range %	91-100
1:800	Average %	93
	Range %	87-98

Recovery

The recovery of rat HNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-95
EDTA plasma (n=4)	98	94-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1000	0.118	0.110	0.114
500	0.184	0.192	0.188
250	0.265	0.273	0.269
125	0.474	0.466	0.470
62.5	0.625	0.615	0.620
31.2	1.129	1.074	1.102
15.6	2.182	2.281	2.232
0	2.877	2.985	2.931

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Rat HNE ELISA Kit was designed for the quantitative measurement of Rat HNE protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 7.8 pg/mL.

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Alda-1 Prevents Pulmonary Epithelial Barrier Dysfunction following Severe Hemorrhagic Shock through Clearance of Reactive Aldehydes Tianfeng Hua, et al,Biomed Research International,2019
Titanium Dioxide Nanoparticles Provoke Testicular Injury and Apoptosis in Sperm through Oxidative Stress-Activated Mitochondrial and Death Receptor Pathways Mohamed A El-Missiry, et al,Imperial Journal Of Interdisciplinary Research,2019
Bone turnover and oxidative stress markers in estrogendeficient rats treated with Marantodes pumilum leaves and roots: A comparative study Tijjani Rabiu Giaze.et al,TROP J PHARM RES,/
Titanium Dioxide Nanoparticles Provoke Testicular Injury and Apoptosis in Sperm through Oxidative Stress-Activated Mitochondrial and Death Receptor Pathways Mohamed A El-Missiry.et al,Imperial Journal of Interdisciplinary Research ,2017
Age-dependent changes of the antioxidant system in rat liver are accompanied by altered MAPK activation and aDecline in mTOR signaling Wei Yang. et al,EXCLI Journal ,2015

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.