Human Angiopoietin-related protein 7(ANGPTL7) ELISA kit

Code CSB-EL001715HU
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human ANGPTL7 ELISA Kit was designed for the quantitative measurement of Human ANGPTL7 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Target Name angiopoietin-like 7
Alternative Names Angiopoietin like 7 ELISA Kit; Angiopoietin like factor (CDT6) ELISA Kit; Angiopoietin like factor ELISA Kit; Angiopoietin related protein 7 ELISA Kit; Angiopoietin-like factor ELISA Kit; Angiopoietin-like protein 7 ELISA Kit; Angiopoietin-related protein 7 ELISA Kit; ANGL7_HUMAN ELISA Kit; ANGPTL7 ELISA Kit; AngX ELISA Kit; CDT6 ELISA Kit; Cornea derived transcript 6 protein ELISA Kit; Cornea-derived transcript 6 protein ELISA Kit; dJ647M16.1 ELISA Kit; OTTHUMP00000001986 ELISA Kit; RP4-647M16.2 ELISA Kit
Abbreviation ANGPTL7
Uniprot No. O43827
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ANGPTL7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 99
Range % 92-104
1:2 Average % 87
Range % 82-93
1:4 Average % 92
Range % 87-98
1:8 Average % 101
Range % 98-105
Recovery
The recovery of human ANGPTL7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 103 92-106
EDTA plasma (n=4) 95 89-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 1.942 2.042 1.992 1.881
10 1.239 1.149 1.194 1.083
5 0.759 0.719 0.739 0.628
2.5 0.453 0.464 0.459 0.348
1.25 0.311 0.321 0.316 0.205
0.625 0.212 0.223 0.218 0.107
0.312 0.161 0.165 0.163 0.052
0 0.110 0.111 0.111  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Has a role in the formation and organization of the extracellular matrix. In the eye, it functions as a mediator of dexamethasone-induced matrix deposition in the trabecular meshwork, the tissue responsible for the outflow of the ocular aqueous humor and for the maintenance of intraocular pressure. Is a negative regulator of angiogenesis in the cornea, and plays a major role in maintaining corneal avascularity and transparency.
Gene References into Functions
  1. obesity increases the level of ANGPTL7 in both plasma and adipose tissue. Increased expression of ANGPTL7 might play a minor role in the regulation of TG level in obese subjects either directly or through interaction with other ANGPTL protein members. Physical exercise reduced the level of ANGPTL7 highlighting the potential for targeting this protein as a therapeutic target for regulating dyslipidemia. PMID: 28264047
  2. ANGPTL7, which is abundantly expressed in keratocytes, plays a major role in maintaining corneal avascularity and transparency. PMID: 25622036
  3. ANGPTL7 is markedly down regulated in metastatic liver cancer. PMID: 25854806
  4. Angiopoietin-like 7 is a novel pro-angiogenetic factor over-expressed in cancer PMID: 24903490
  5. Together with previous findings, these properties strengthen ANGPTL7's candidacy for the regulation of intraocular pressure and glaucoma. PMID: 21199193
  6. Induction of ANGPTL7 secretion by glaucoma stimuli and increased concentration of ANGPTL7 in glaucomatous AH suggest that ANGPTL7 is overexpressed in glaucoma. PMID: 18421092

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Subcellular Location Secreted.
Tissue Specificity Higly expressed in the cornea (at protein level). Expression is restricted to the stromal layer. Also detected at the junction between the corneal stromal layer and the conjuctiva. Not detected in the sclera.
Database Links

HGNC: 24078

KEGG: hsa:10218

STRING: 9606.ENSP00000366015

UniGene: Hs.146559

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