Human Annexin Ⅰ(ANX-Ⅰ) ELISA Kit

Instructions
Code CSB-E12155h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name annexin A1
Alternative Names Annexin 1 ELISA Kit; Annexin A1 ELISA Kit; Annexin I (lipocortin I) ELISA Kit; Annexin I ELISA Kit; Annexin-1 ELISA Kit; AnnexinA1 ELISA Kit; AnnexinI ELISA Kit; ANX 1 ELISA Kit; ANX A1 ELISA Kit; ANX1 ELISA Kit; ANXA 1 ELISA Kit; ANXA1 ELISA Kit; ANXA1 protein ELISA Kit; ANXA1_HUMAN ELISA Kit; Calpactin 2 ELISA Kit; Calpactin II ELISA Kit; Calpactin-2 ELISA Kit; CalpactinII ELISA Kit; Chromobindin 9 ELISA Kit; Chromobindin-9 ELISA Kit; Chromobindin9 ELISA Kit; HGNC:533 ELISA Kit; Lipocortin 1 ELISA Kit; Lipocortin I ELISA Kit; Lipocortin1 ELISA Kit; LipocortinI ELISA Kit; LPC 1 ELISA Kit; LPC1 ELISA Kit; p35 ELISA Kit; Phospholipase A2 inhibitory protein ELISA Kit
Abbreviation ANXA1
Uniprot No. P04083
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ANX-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %81-93
1:2Average %95
Range %90-100
1:4Average %98
Range %91-106
1:8Average %92
Range %80-98
Recovery
The recovery of human ANX-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9280-110
EDTA plasma (n=4)9691-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
201.958 1.872 1.915 1.772
101.794 1.696 1.745 1.602
51.405 1.384 1.395 1.252
2.50.887 0.839 0.863 0.720
1.250.534 0.524 0.529 0.386
0.6250.342 0.319 0.331 0.188
0.3120.236 0.219 0.228 0.085
00.149 0.137 0.143  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity
Gene References into Functions
  1. Results show high expression of AnxA1 in triple-negative breast cancer (TNBC) and in lymph node metastasis. A positive correlation is observed between the expression levels of AnxA1 and its receptor, FPR1 in primary tumors. The autocrine activation of FPR1 by AnxA1 might be a pivotal target for TNBC. PMID: 29932988
  2. These results could lead to further information on ANXA1 intracellular role in pancreatic cancer , explaining other aspects that are apart from its tumorigenic behaviour. PMID: 29986379
  3. There was an inverse correlation between the expressions of miR-196a and ANXA1 in serum (Pearson's correlation of -0.54, P = 0.021). Our data revealed that the expression of serum ANXA1 in esophageal squamous cell carcinoma (ESCC) patients increases after chemoradiotherapy and the increased fold change in serum ANXA1 confers independent negative prognostic impact in ESCC. PMID: 30249885
  4. the results highlighted the protective effects of ANXA1 on bronchial epithelium injury, which most likely occurred via the PTEN/FAK/PI3K/Akt signaling pathway. Thus, the present study contributes to a potential therapeutic strategy for asthma patients. PMID: 29749523
  5. Enhanced levels of Annexin A1 were found in the serum and adipose tissue of individuals with obesity and type 2 diabetes mellitus. PMID: 29677533
  6. Annexin A1 expression is upregulated in patients with COPD and affects lung fibroblast function. PMID: 29440885
  7. that the actions of ANXA1 in the inflammatory and neoplastic processes of the esophagus and stomach are specifically mediated by the formylated peptides receptor 1 PMID: 29254791
  8. Annexin A1 has a similar immunogenic expression and correlation with its analog Annexin A2 and their association may be a novel immune target of BD in Han Chinese population PMID: 28963375
  9. We further identified this underlying mechanism also involved a PPARgamma-induced ANXA1-dependent autoubiquitination of cIAP1, the direct E3 ligase of RIP1, shifting cIAP1 toward proteosomal degradation..our study provides first insight for the suitability of using drug-induced expression of ANXA1 as a new player in RIP1-induced death machinery in triple-negative breast cancer PMID: 29021293
  10. this review details the therapeutic relevance of ANXA1 and its derived peptides in cardiovascular diseases since atherosclerosis stands out as a chronic inflammatory disease with impaired resolution and continuous leukocyte recruitment. PMID: 27860536
  11. Our results indicated that ANXA1 may be a key mediator of hypoxia-related metastasis-associated processes in prostate cancer PMID: 27834582
  12. Data indicate a regulatory circuit between ANXA1, NF-kB, c-myc and miR-196a which regulates breast cancer cell proliferation and tumor growth. PMID: 27105503
  13. In colorectal cancer, miR-196a over-expression was negatively correlated with annexin A1 protein expression (r = -0.738, p < 0.001), and both were indicators of unfavorable prognosis in terms of poor differentiation, larger tumor size, and advanced clinical stage PMID: 29091952
  14. The data of this study supported a potential role of ANXAl in AD by reducing Abeta levels and decreasing neuroinflammation, suggesting a novel view that ANXAl may play a protective role in AD progression. PMID: 27590054
  15. these data indicate that AnxA1 is actively expressed during L. braziliensis infection. PMID: 28289158
  16. Data, including data from studies using knockout mice, suggest cAMP-elevating agents increase levels of ANXA1; this is involved in role of cAMP in resolving acute inflammation (here, ability of cAMP-elevating agents to treat LPS-induced neutrophilic pleurisy). These results reinforce hypothesis that ANXA1 acts in multiple cell types (neutrophils, macrophages) and at multiple levels to promote resolution of inflammation. PMID: 28655761
  17. Circulating IgG antibodies to ANXA1 could be used as a biomarker for early diagnosis of non-small cell lung carcinoma. PMID: 28551657
  18. Down-regulation of Annexin A1 in nasopharyngeal carcinoma may lead to the overexpression of S100A9/Vimentin, which may increase the possibility of the invasion ability of NPC cells by adjusting the function of cytoskeleton proteins. PMID: 28355254
  19. ANXA1 is down-regulated and differentially expressed within the SCD genotypes PMID: 27802331
  20. Annexin A1-regulated contacts function in the transfer of endoplasmic reticulum-derived cholesterol to the multivesicular endosomes/bodies when low-density lipoprotein-cholesterol in endosomes is low. PMID: 27270042
  21. This review explores the diverse actions of annexin A1 on breast tumor cells and various host cell types, including stromal immune and structural cells, particularly in the context of cancer immunoediting. [annexin A1] PMID: 28212890
  22. ANXA1 restores Abeta42-induced blood brain barrier disruption through inhibition of RhoA-ROCK signaling pathway PMID: 27633771
  23. High ANXA1 expression is associated with lymphatic invasion and malignant progression of lung cancer. PMID: 28009433
  24. Annexin A1 role in colon cancer cells drug resistance to 5-fluorouracil PMID: 27840982
  25. ANXA1 promotes the proliferation of Eca109 cells, and increases the expression of Snail, whereas it inhibits that of E-cadherin, thus enhancing the migration and invasion of ESCC cells. miRNA-196a negatively regulates the expression of ANXA1 PMID: 28035369
  26. the present study provided evidence suggesting that ANXA1 may contribute to the growth and invasion of NSCLC cell lines PMID: 27035116
  27. Simultaneous knock-down of HIF-1alpha and Annexin A1 (ANXA1), one of the identified genes, resulted in complete cessation of proliferation of gastric cancer. PMID: 26760764
  28. ANXA1 and CALD1 proteins are independent markers for tamoxifen therapy outcome and are associated to fast tumor progression. PMID: 26657294
  29. High ANXA1 expression is associated with Colorectal Cancer. PMID: 26687139
  30. Results show that ANXA1 and ANXA10 are highly expressed in pancreatic ductal adenocarcinoma and its metastases to the liver comparing to intrahepatic cholangiocellular carcinoma. PMID: 26644413
  31. We conclude that ANX-A1 is an important regulator of mast cell reactivity to compound 48/80 exerting a negative feedback effect through a mechanism that depends at least partly on the FPR receptor PMID: 26803520
  32. AnxA1 and its mimetic peptides inhibit neutrophil tissue accumulation by reducing leukocyte infiltration and activating neutrophil apoptosis. PMID: 26885535
  33. results suggest that overexpression of CRISP-3 in prostate tumor may maintain higher PSA expression and lower ANXA1 expression PMID: 26369530
  34. Data show that high density lipoprotein (HDL) up-regulated expression of annexin A1 (ANXA1) in HUVEC vascular endothelial cells in a dose-dependent (Fig. 1A-B) and time-dependent manners. PMID: 27012521
  35. Annexin A1 is involved in the acquisition and maintenance of a stem cell-like/aggressive phenotype in prostate cancer cells with acquired resistance to zoledronic acid. PMID: 26312765
  36. increased AnxA1 levels were associated with a systemic inflammatory phenotype in preeclampsia. PMID: 26398190
  37. Data show that elevated levels of annexin A1 are associated with small cell lung cancer {SCLC) brain metastasis. PMID: 26135980
  38. Within the basal subtype of breast cancer, patients show significantly poorer overall survival associated with higher expression of annexin A1. PMID: 26000884
  39. ANXA1 negatively regulated the step of viral RNA replication rather than that of viral entry in human hepatocytes. PMID: 25899628
  40. Loss of ANXA1 is associated with lymphatic metastasis in pancreatic ductal adenocarcinoma. PMID: 25854353
  41. ANXA1 is overexpressed in familial breast cancer patients with BRCA1/2 mutations and correlated with poor prognosis features: triple negative and poorly differentiated tumors. PMID: 26137966
  42. Cardioprotective potential of annexin-A1 mimetics in myocardial infarction PMID: 25460034
  43. data indicate the involvement of ANXA1Ac2-26 in the altered expression of genes involved in tumorigenic processes, which could potentially be applied as a therapeutic indicator of cervical cancer PMID: 26072160
  44. These results suggested that ANXA1, which enhanced breast cancer invasion and metastasis under hypoxia, were significantly associated with the worst patient outcome in triple-negative breast cancer PMID: 25592491
  45. Increased expression of ANXA1 protein associates with aggressive progression and poor prognosis in hepatocellular carcinoma patients. PMID: 25412936
  46. Overexpression of ANXA1 induced by low-concentration Arsenic Trioxide (ATO) makes cancer cells more resistant to the agent via activated ERK MAPKs. Specific silencing of ANXA1 increased the sensitivity of cancer cells to ATO treatment. PMID: 25983101
  47. ANXA1 may contribute to the regulation of tumor growth and metastasis through paracrine mechanisms that are mediated by FPR2/ALX PMID: 25490767
  48. the co-upregulated expression of mast cell chymase and ANXA1-FPR1 system in ectopic endometrium suggests their involvement in the development of endometriotic lesions. PMID: 25201101
  49. This data suggests that ANXA1-regulated miR26b* and miR562 may play a role in wound healing and tumor-induced endothelial cell tube formation by targeting NF-kappaB expression and point towards a potential therapeutic target for breast cancer. PMID: 25536365
  50. Study provides new insights on the role of ANXA1 protein in pancreatic cancer progression and suggest that ANXA1 protein could regulate metastasis by favouring cell migration/invasion. PMID: 25510623
  51. These findings establish a central role for ANXA1 in the cell migration through the activation of NFkappaB, ERK1/2 and RhoA. PMID: 25866182
  52. Identify signaling pathways involved in annexin A1 pro-invasive role and suggest that externalized full-length annexin A1 interacts with formyl peptide receptors in a juxtacrine manner while ANXA 2-28 release allows autocrine and paracrine interaction. PMID: 25362540
  53. ANXA1 may become a promising marker to predict the recurrence and drug resistance of bladder cancer and provide guidance for surveillance. PMID: 25337195
  54. The expression levels of Hsp90-beta and annexin A1 positively correlated and such co-overexpression of Hsp90-beta and annexin A1 contributed to lung cancer diagnosis. PMID: 25300907
  55. Annexin A1 may be a potential biomarker for the development and prognosis of NPC. PMID: 25322804
  56. This work suggests that circulating IgG for ANXA1-derived peptide antigens may have both diagnostic and prognostic values for breast cancer although further screening is needed to identify more such peptide antigens derived from tumor-associated antigens. PMID: 25344217
  57. The broken dialog between ANXA1 and CK18 in normal breast tissues may play a critical role in breast cancer development. PMID: 25028131
  58. Patients with active inflammatory bowel disease had elevated levels of secreted ANXA1-containing exosomes in sera. PMID: 25664854
  59. Data revealed important functions of ANXA1 in tumorigenesis and cancer development. PMID: 25038797
  60. These results demonstrate that a necroptosis pathway, likely mediated by annexin 1 acting through the FPR1 receptor, contributes to Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis. PMID: 25031270
  61. AnxA1 participates in a paradigm in which malignant prostate epithelial cells that are not cancer stem cells are induced to gain cancer stem cell-like properties. PMID: 24464914
  62. Urinary ANXA1 protein is detectable glomerulopathy patients except those with minimal change disease. PMID: 24591769
  63. high expression of ANXA1 is associated with poor therapeutic response and adverse outcomes in rectal cancer patients treated with neoadjuvant concurrent chemoradiation therapy (CCRT). PMID: 24810927
  64. These data show a multiantibody composition in lupus nephritis, where IgG2 autoantibodies against alpha-enolase and annexin AI predominate in the glomerulus and can be detected in serum. PMID: 24790181
  65. result of the present study suggest that ANXA1 complex coordinates the actions of PKA and PKC to control the distribution of oxytocin vesicles between the axon and the cell body PMID: 24118254
  66. both the AnxA1 and Gal-1 anti-inflammatory proteins are deregulated in precancerous gastric lesions PMID: 24719523
  67. Propose a novel mechanism to explain the antiinflammatory actions of cromones on neutrophil trafficking involving annexin A1. PMID: 20558817
  68. In the current study, we review the important biological functions of annexin A1 in different tumors. PMID: 24242295
  69. The expression levels of three putative HA-regulated proteins (TALDO, ANXA1 and EF2) in control, H2O2-, HA- and HA/H2O2-treated OA chondrocytes were verified by Western blotting and the results indeed support the notion that HA acts in anti-oxidation PMID: 24480285
  70. nuclear ANXA1 may be potentially used as a prognostic biomarker for esophageal squamous cell carcinoma. PMID: 24551299
  71. Annexin1 was abundantly detected in each epidermal layer in lupus lesional skin. Additionally, anti-annexin1-IgG was higher in SLE patients with arthritis and negatively correlated with white blood cells (WBC). PMID: 24300781
  72. Loss of ANXA1 expression may support inflammation during IBD and can serve as a biomarker of disease progression PMID: 24130820
  73. findings provide both clinical and mechanistic evidences that FoxM1 contributes to glioma development by directly up-regulating Anxa1 expression PMID: 23991102
  74. annexin A1 has important anti-inflammatory effects in rheumatoid arthritis (RA).Patients with RA had reduced peripheral blood monocyte expression of annexin A1 in response to glucocorticoids compared with healthy control individuals. PMID: 23958797
  75. Anxa1 might specifically function either as a tumor suppressor or a tumor promoter candidate for certain cancers. [Review] PMID: 24156336
  76. This is the first study to assess lipoxin A4 and annexin A1 levels in wheezy infants. PMID: 24525704
  77. Upregulated ANXA1 expression may be associated with carcinogenesis, progression, invasion and metastasis of gastric adenocarcinoma. PMID: 24282368
  78. These results show for the first time that SUMOylation can be regulated by an external signal (EGF) and indicate the presence of a cross-talk between the N-terminal and C-terminal domains of ANXA1 through post-translational modifications. PMID: 23727357
  79. Used proteomics to study Annexin A1 (Anxa1) interacting proteins in the Philadelphia-positive KCL22 cell line. PMID: 23754495
  80. Suggest annexin A1 may serve as a new immunohistochemical marker of cholangiocarcinoma. PMID: 23674846
  81. data support a significant correlation between Annexin A1 expression and pathological differentiation grade, and a functional role of Annexin A1 in inhibiting cell proliferation and cell differentiation in OSCC PMID: 23403091
  82. Annexin A1 is exposed on the surface of early apoptotic cells and suppresses CD8+ T cell immunity. PMID: 23638088
  83. Annexin A1 expression can potentially be used as a predictive biomarker to select Ooral squamous cell carcinoma patients with moderate/poorly differentiated tumor who may benefit from docetaxel, cisplatin and 5-fluorouracil induction chemotherapy. PMID: 23786757
  84. AnxA1 protein correlates positively with salivary cortisol, indicating that cortisol is most likely a regulator of AnxA1 in human saliva. PMID: 23017499
  85. Annexin A1 down regulation in patients with bladder pain syndrome might decrease cell survival and damage urothelial function. PMID: 23376147
  86. Results have provided evidence that galectin-1 and mainly annexin-A1 are overexpressed in both gastritis and gastric cancer, suggesting a strong association of these proteins with chronic gastric inflammation and carcinogenesis. PMID: 23431236
  87. Up-regulation of annexin A1 gene expression and protein points to this protein as a biomarker for transverse (T)-tubular repair. PMID: 23413360
  88. Overexpression of ANXA1 might be mediated by human papillomavirus (HPV) E6 in penile squamous cell carcinoma of patients with high-risk HPVs. PMID: 23341933
  89. Annexin A1 is involved in reducing the production of pro-inflammatory mediators in smooth muscle cells derived from human atherosclerotic plaques. PMID: 23154128
  90. Depletion of annexin A1 or A2 alters the retrograde transport of shiga toxin but not ricin, without affecting toxin binding or internalization. PMID: 22792315
  91. N- terminal peptide of Annexin A1 Ac2-26 is able to stimulate direct migration of WS1 cells in high glucose treatment possibly due to the increased receptor expression observed in hyperglycemia conditions PMID: 23029153
  92. detection of a selective loss of ANXA1 in the plasma and cerebrovascular endothelium of patients with multiple sclerosis PMID: 23277546
  93. a novel epithelial FPR1/NOX1-dependent redox signaling pathway that promotes mucosal wound repair. PMID: 23241962
  94. A novel role for ATM kinase activity in the dynamic exchange of proteins in dna damage response complexes and identifies a role for ANXA1 in cellular radioprotection. PMID: 22909323
  95. The upregulation of annexin A1 was associated with poor post-surgical survival time and lymphatic metastasis of lung cancer patients PMID: 22929401
  96. The data suggested that in lung fibroblasts annexin A1 regulates TNF-induced proliferation and inflammatory responses, which depend on formyl-peptide receptor 2. Annexin A1 also mediates the expression of glucocorticoid-induced leucine zipper. PMID: 22777765
  97. VEGF-induced decrease of miR-196a expression may participate to the angiogenic switch by maintaining the expression of ANXA1 to levels required to enable p38-ANXA1-dependent endothelial cell migration and angiogenesis in response to VEGF. PMID: 22773844
  98. Endothelial microparticles are incorporated by endothelial cells in an annexin I/phosphatidylserine receptor-dependent manner and protect target cells against apoptosis. PMID: 22701020
  99. We conclude that biologically active AnxA1 may play a role in the anti-inflammatory properties of ATRA-treated APL cells during the process of granulocytic differentiation. PMID: 22378018
  100. N-terminal region was dispensable for AnxA1-induced FPR2/ALX down-regulation in both the homologous and heterologous desensitization modes. PMID: 22610094

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Subcellular Location Nucleus, Cytoplasm, Cell projection, cilium, Cell membrane, Membrane, Peripheral membrane protein, Endosome membrane, Peripheral membrane protein, Basolateral cell membrane, Apical cell membrane, Lateral cell membrane, Secreted, Secreted, extracellular space, Cell membrane, Peripheral membrane protein, Extracellular side, Secreted, exosome, Cytoplasmic vesicle, secretory vesicle lumen, Cell projection, phagocytic cup, Early endosome, Cytoplasmic vesicle membrane, Peripheral membrane protein
Protein Families Annexin family
Tissue Specificity Detected in resting neutrophils (PubMed:10772777). Detected in peripheral blood T-cells (PubMed:17008549). Detected in extracellular vesicles in blood serum from patients with inflammatory bowel disease, but not in serum from healthy donors (PubMed:256648
Database Links

HGNC: 533

OMIM: 151690

KEGG: hsa:301

STRING: 9606.ENSP00000257497

UniGene: Hs.494173

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