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| Code | CSB-E12156h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human ANX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:8 | Average % | 103 | ||||
| Range % | 96-110 | |||||
| The recovery of human ANX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The ELISA Kit is designed to measure human ANXA2 levels in samples, including serum, urine, saliva, or cell lysates. It uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of ANXA2 in samples. The color intensity is measured at 450 nm via a microplate reader.
ANXA2, a calcium-regulated phospholipid-binding protein, is involved in several critical cellular processes, such as cell proliferation, cell adhesion, cell motility, angiogenesis, vesicular transport, exocytosis, and endocytosis. It is abundantly expressed in multiple cancers, including breast cancer, squamous cervical cancer, pancreatic ductal adenocarcinoma (PDA), hepatocellular carcinoma (HCC), and lung cancer. ANXA2 plays a pivotal role in cancer cell proliferation, migration, invasion, metastasis, epithelial-mesenchymal transition (EMT), and treatment resistance. Abnormal expression of ANXA2 can be used as a potential predictive factor, diagnostic biomarker, and therapeutic target in cancer therapy.
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