Human Apolipoprotein C-IV(APOC4) ELISA kit

Code CSB-EL001934HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human APOC4 ELISA Kit was designed for the quantitative measurement of Human APOC4 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL .

Target Name apolipoprotein C-IV
Alternative Names APO C4 ELISA Kit; Apo CIV ELISA Kit; Apo-CIV ELISA Kit; APOC 4 ELISA Kit; ApoC IV ELISA Kit; ApoC-IV ELISA Kit; APOC4 ELISA Kit; APOC4_HUMAN ELISA Kit; Apolipoprotein C IV ELISA Kit; Apolipoprotein C-IV ELISA Kit; Apolipoprotein C4 ELISA Kit
Abbreviation APOC4
Uniprot No. P55056
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 ng/mL-400 ng/mL
Sensitivity 1.56 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human APOC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:200 Average % 92  
Range % 88-96  
1:400 Average % 87  
Range % 84-90  
1:800 Average % 95  
Range % 91-99  
1:1600 Average % 97  
Range % 92-102  
Recovery
The recovery of human APOC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-98  
EDTA plasma (n=4) 98 94-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
400 0.080 0.083 0.082    
200 0.117 0.119 0.118    
100 0.177 0.172 0.175    
50 0.276 0.296 0.286    
25 0.504 0.490 0.497    
12.5 0.923 0.931 0.927    
6.25 1.461 1.496 1.479    
0 2.539 2.587 2.563    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
May participate in lipoprotein metabolism.
Gene References into Functions
  1. APOC4 rs1132899 polymorphism was associated with an increased risk of premature coronary artery disease in Chinese subjects. PMID: 26129832
  2. ApoC-IV overexpression may perturb lipid metabolism leading to lipid accumulation. HCV core protein may modulate ApoC-IV expression through Ku antigen and PPARgamma/RXRalpha complex. PMID: 18809223
Subcellular Location Secreted.
Protein Families Apolipoprotein C4 family
Tissue Specificity Expressed by the liver and secreted in plasma.
Database Links

HGNC: 611

OMIM: 600745

KEGG: hsa:346

STRING: 9606.ENSP00000468236

UniGene: Hs.731599

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