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Human Apolipoprotein O(APOO) ELISA kit

Human Apolipoprotein O(APOO) ELISA kit

Protocol

Code	CSB-EL001948HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

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Target Background

Product Details

Target Name

apolipoprotein O

Alternative Names

APOO; FAM121B; MIC23; MIC26; My025; UNQ1866/PRO4302; MICOS complex subunit MIC26; Apolipoprotein O; MICOS complex subunit MIC23; Protein FAM121B

Abbreviation

APOO

Uniprot No.

Q9BUR5

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.08 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human APOO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	102
	Range %	97-106
1:200	Average %	86
	Range %	83-91
1:400	Average %	108
	Range %	104-112
1:800	Average %	85
	Range %	81-90

Recovery

The recovery of human APOO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	97-106
EDTA plasma (n=4)	89	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
20	0.122	0.126	0.124
10	0.207	0.215	0.211
5	0.326	0.331	0.329
2.5	0.553	0.549	0.551
1.25	0.846	0.854	0.850
0.625	1.197	1.200	1.199
0.312	1.586	1.598	1.592
0	2.387	2.400	2.394

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human APOO ELISA Kit was designed for the quantitative measurement of Human APOO protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.08 ng/mL.

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Target Background

Function
(From Uniprot)

Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays a crucial role in crista junction formation and mitochondrial function. Can promote cardiac lipotoxicity by enhancing mitochondrial respiration and fatty acid metabolism in cardiac myoblasts. Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL.

Gene References into Functions

The human apolipoprotein MIC26 is a bona fide subunit of the MICOS complex. PMID: 25764979
It is likely that apoO participates in fatty acid metabolism and the inflammatory response in HepG2 cells. PMID: 24341743
APOO represents a link between impaired mitochondrial function and cardiomyopathy onset, and targeting APOO-dependent metabolic remodeling has potential as a strategy to adjust heart metabolism and protect the myocardium from impaired contractility. PMID: 24743151
relationships between plasma apoO levels and high-sensitive C-reactive protein (hs-CRP) levels, as well as other lipid parameters in healthy subjects and patients with established acute coronary syndrome PMID: 22693255
apolipoprotein O is the first chondroitin sulfate chain containing apolipoprotein PMID: 16956892

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Subcellular Location

Mitochondrion inner membrane; Single-pass membrane protein. Secreted. Mitochondrion. Golgi apparatus membrane. Endoplasmic reticulum membrane.

Protein Families

Apolipoprotein O/MICOS complex subunit Mic27 family

Tissue Specificity

Expressed in all tissues examined. Up-regulated in diabetic heart.

Database Links

HGNC: 28727

OMIM: 300753

KEGG: hsa:79135

STRING: 9606.ENSP00000368528

UniGene: Hs.495851

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Human Apolipoprotein O(APOO) ELISA kit

Product Details

Related Products

Customer Reviews and Q&A

Target Background