Human B cell activation factor,BAFF ELISA Kit

Instructions
Code CSB-E11912h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name tumor necrosis factor (ligand) superfamily, member 13b
Alternative Names ApoL related ligand TALL 1 ELISA Kit; B cell Activating Factor ELISA Kit; B lymphocyte stimulator ELISA Kit; B-cell-activating factor ELISA Kit; BAFF ELISA Kit; BLyS ELISA Kit; CD 257 ELISA Kit; CD257 ELISA Kit; CD257 antigen ELISA Kit; Delta BAFF ELISA Kit; Dendritic cell derived TNF like molecule ELISA Kit; Dendritic cell-derived TNF-like molecule ELISA Kit; DTL ELISA Kit; DTL precursor ELISA Kit; PRO738 ELISA Kit; soluble form ELISA Kit; TALL 1 ELISA Kit; TALL-1 ELISA Kit; TALL1 ELISA Kit; THANK ELISA Kit; TN13B_HUMAN ELISA Kit; TNF and APOL related leukocyte expressed ligand 1 ELISA Kit; TNF homolog that activates apoptosis ELISA Kit; TNF homolog that activates apoptosis NKFB and JNK. ELISA Kit; TNF- and APOL-related leukocyte expressed ligand 1 ELISA Kit; TNFSF13B ELISA Kit; TNFSF20 ELISA Kit; TNLG7A ELISA Kit; Tumor necrosis factor (ligand) superfamily member 13b ELISA Kit; Tumor necrosis factor ligand 7A ELISA Kit; Tumor necrosis factor ligand superfamily member 13b ELISA Kit; Tumor necrosis factor ligand superfamily member 20 ELISA Kit; Tumor necrosis factor like protein ZTNF4 ELISA Kit; Tumor necrosis factor superfamily member 13B ELISA Kit; UNQ401 ELISA Kit; ZTNF4 ELISA Kit
Abbreviation TNFSF13B
Uniprot No. Q9Y275
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human BAFF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %82-95
1:2Average %90
Range %85-96
1:4Average %93
Range %90-95
1:8Average %92
Range %88-96
Recovery
The recovery of human BAFF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9285-99
EDTA plasma (n=4)9087-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.912 2.953 2.933 2.819
102.142 2.175 2.159 2.045
51.295 1.312 1.304 1.190
2.50.727 0.695 0.711 0.597
1.250.426 0.443 0.435 0.321
0.6250.297 0.312 0.305 0.191
0.3120.247 0.261 0.254 0.140
00.112 0.115 0.114  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
Gene References into Functions
  1. BAFF loop region controls B cell survival and regulates recognition by different inhibitors PMID: 29572442
  2. analysis of how BAFF is neutralized by belimumab gives insight into treatment of systemic lupus erythematosus PMID: 29572471
  3. Study uncovers a previously unrecognized regulation of BAFF by testosterone and raises important questions about BAFF in testosterone-mediated protection against autoimmunity. Among healthy men, serum BAFF levels are higher in men with low testosterone. PMID: 29802242
  4. High BAFF expression is associated with systemic lupus erythematosus. PMID: 28992184
  5. Co-immunoprecipitation analysis and siRNA-mediated suppression of CREB expression indicated that phospho-CREB has a positive effect on pro-inflammatory gene expression in the crosstalk between BAFF- and TLR4-mediated signaling by forming trimeric complexes containing NF-kappaB, CBP, and CREB PMID: 28374824
  6. this study shows that BAFF augments IgA2 and IL-10 production by TLR7/8 stimulated total peripheral blood B cells PMID: 28921509
  7. elevated pretransplant serum BAFF levels negatively affect renal allograft survival and represent a risk factor for allosensitization and subsequent antibody-mediated rejection PMID: 29277566
  8. Increased BAFF expression is associated with B cell class switching in patients with tuberculous pleural effusion. PMID: 29845274
  9. Expression patterns of BAFF and its receptors differ according to lupus nephritis class. PMID: 29087261
  10. Serum BAFF levels are elevated in idiopathic inflammatory myositis, more so in children. PMID: 29516280
  11. Post-transplant antibody mediated rejection in kidney transplantation recipients can be predicted by perioperative elevations in serum BAFF level. PMID: 27888573
  12. The results of the present study revealed a correlation between BAFF and the PI3K/Akt/mTOR signaling pathway, and it is hypothesized that they are involved in the pathogenesis of lupus nephritis PMID: 28849060
  13. The results suggest that increased levels of BAFF and APRIL produced in the central nervous system may influence the development of anti-neutrophil cytoplasmic antibody-hypertrophic pachymeningitis. PMID: 28847534
  14. Blood B lymphocyte stimulator (BLyS)/BAFF levels of HIV-uninfected commercial sex workers (CSWs) were lower than those observed in both HIV-infected CSW and HIV-uninfected non-CSW groups. PMID: 27561453
  15. our study throws light on the crosstalk between BAFF and BCR signaling pathways in neoplastic B cells, and provides insights into the mechanistic effects of SYK inhibitors in CLL. PMID: 28838991
  16. genetic polymorphisms of BAFF may increase the risk of posttransplant development of donor specific antibodies in kidney allograft recipients PMID: 28624489
  17. P\pre-sensitized patients had significantly higher BAFF levels before transplantation and suffered significantly more often from early steroid-resistant, mainly antibody-mediated rejections PMID: 28867309
  18. Elevated blood BAFF levels could be associated with a more stable disease. PMID: 27383531
  19. BAFF rs9514828 polymorphism may be associated with the chronic hepatitis and the combinatorial action of rs9514828 and rs12583006 may confer susceptibility to chronic HBV infection and the resolution of the infection, suggesting that host genetic factors associated with B cell mediated immune responses are involved in chronic HBV infection. PMID: 28627389
  20. This study demonstrated that An Increase of Cerebrospinal Fluid B-cell Activating Factor Level in Pediatric Patients With Acute Viral Encephalitis. PMID: 28259511
  21. data show that BAFF levels at the time of cGvHD diagnosis are associated with non-relapse-mortality, and also are potentially useful for risk stratification. PMID: 28481353
  22. BAFF-R was consistently expressed on B cells infected by HCMV. Enhancement of BAFF/BAFF-R signaling decreased the apoptosis rate and extended the survival of B cells. PMID: 28442365
  23. soluble BCMA sequesters circulating BAFF, thereby preventing it from performing its signaling to stimulate normal B-cell and plasma cell development, resulting in reduced polyclonal antibody levels in multiple myeloma patients. PMID: 26960399
  24. up-regulated expression in intractable temporal lobe epilepsy PMID: 28441631
  25. results showed that serum BAFF in nasal type, extranodal NK/T cell lymphoma patients was significantly higher than that in control group and negatively correlated with patients' survival. PMID: 27668971
  26. BAFF has a role in inducing IL35 production by regulatory B cells in lupus PMID: 28844943
  27. Inhibition of ADAM10 augments BAFF-dependent survival of primary human B cells, whereas inhibition of ADAM17 increases BAFFR expression levels PMID: 28249164
  28. Data presented show that B-cell activating factor (BAFF) plays a central role in the induction and maintenance of cigarette smoke-induced pulmonary antinuclear antibodies and suggest a therapeutic potential for BAFF blockade in limiting autoimmune processes associated with smoking. PMID: 28039405
  29. Among the BAFF receptors in a cohort of rheumatoid arthritis (RA) patients, the AA have shown, by fluorescence activated cell sorter (FACS) analysis of median fluorescence intensity (MFI), that transmembrane activator and calcium-modulating cyclophilin ligand interactor (TACI) and B cell maturation antigen (BCMA) do not change PMID: 28834574
  30. The BAFF promoter increased in response to TNF-alpha treatment or overexpression of HIF-1alpha. However, TNF-alpha-induced BAFF expression and promoter activity decreased after treatment with the ERK inhibitor PD98059. PMID: 28383556
  31. This study indicates that orbital fibroblasts from Graves' orbitopathy can express BAFF and mediate the intraorbital survival of B cells via BAFF mechanism. PMID: 28087387
  32. Rapamycin attenuates excessive hsBAFF-induced cell proliferation/survival via blocking mTORC1/2 signaling in normal and neoplastic B-lymphoid cells. PMID: 28300280
  33. Inhibition of B cell plasmablast differentiation by reduction of Aiolos and Ikaros may have utility in the treatment of systemic lupus erythematosus , where elevated levels of BAFF and Aiolos may prime CD27(+) memory and double negative memory-like B cells to become Ab-producing plasmablasts in the presence of BAFF and proinflammatory cytokines. PMID: 28848067
  34. BCMA has other contributors for ligands binding except DxL motif. The affinity of BCMA for APRIL higher than for BAFF may be caused by the segment outside of the conservative DxL motif. Moreover, the exposition of new binding modes of BCMA2 interacting with APRIL may establish the foundation of designing novel drugs in the future PMID: 28260502
  35. study demonstrated a high prevalence of endogenous antibodies to BAFF in a multi-ethnic Asian systemic lupus erythematosus (SLE) cohort; while levels of serum BAFF correlated positively with disease activity, levels of anti-BAFF antibody were correlated negatively with levels of its target cytokine, anti-dsDNA antibody and clinical disease activity PMID: 28388832
  36. this study provides new useful information about the increased levels of BAFF observed during HIV-1 infection and highlights the importance of macrophages as a source of BAFF PMID: 27022194
  37. The novel association between BAFF and inflammatory bowel disease (IBD) seems to identify that BAFF might regulate the inflammatory process in these diseases and it appears to be a potential marker of IBD. PMID: 27056038
  38. In BAFF, rs2893321 may be a susceptible genetic variant for the development of GD and AITDs. Associations of rs2893321 with susceptibility to GD and AITDs and the correlation between rs2893321 and TAb exhibit a dimorphic pattern. Additional studies with larger sample sizes are required to confirm our findings. PMID: 27136204
  39. BAFF and IL-17A are associated with different subphenotypes of primary Sjogren's syndrome. PMID: 25941062
  40. The expression levels of serum BAFF and the three receptors (TACI, BCMA and BAFF-R) in non-Hodgkin lymphoma patients were significantly higher than in healthy controls. PMID: 28028945
  41. Plasma BAFF levels were positively associated with serum creatinine, proteinuria, uric acid and group A Streptococcus infection index in patients with IgA nephropathy. PMID: 28260100
  42. this study shows that chicoric acid suppresses BAFF expression by inhibiting NF-kappaB activity, and chicoric acid may serve as a novel therapeutic agent to down-regulate excessive BAFF expression in autoimmune diseases PMID: 28122293
  43. Findings indicate that BAFF expression is significantly increased in chronic rhinosinusitis with nasal polyps patients and may orchestrate inflammatory load in polyp tissues by regulating T and B cell-mediated response. PMID: 28035475
  44. Urinary APRIL (uAPRIL) and BAFF (uBAFF) levels were raised significantly in AN. PMID: 27804111
  45. BAFF levels are lower in patients with antibody-mediated kidney rejection and also in patients with concurrent humoral and cellular rejection compared with patients without rejection PMID: 28083608
  46. The BLyS level is increased in some lupus patients. There was a moderate correlation with titers of anti-DNA antibody and disease activity. PMID: 27100979
  47. In Sjogren's syndrome (SS) patients, EULAR Sjogren's syndrome disease activity index (ESSDAI) is negatively associated with serum levels of 25(OH)-D3 and positively associated with BAFF. PMID: 28074193
  48. These results suggest that miR-202 functions as a modulator that can negatively regulate BAFF by inhibiting multiple myeloma tumor cell survival, growth, and adhesion in the bone marrow microenvironment. PMID: 25971527
  49. Variants in BAFF gene is associated with chronic lymphocytic leukemia. PMID: 27468724
  50. The results demonstrated that BAFF has an important role in the pathogenesis of newly diagnosed childhood immune thrombocytopenia. PMID: 24911453
  51. High levels of serum BAFF were associated with Multiple Myeloma. PMID: 27268602
  52. Expression of BAFF mRNA was not altered in patients with relapsing remitting multiple sclerosis. PMID: 26996115
  53. elevated BLyS levels in patients with systemic lupus erithematosus could be used to identify patients at risk of disease flare. PMID: 26385220
  54. Blood and salivary-gland BAFF-driven B-cell hyperactivity is associated to rituximab inefficacy in primary Sjogren's syndrome PMID: 26688003
  55. expression levels of B cell-activating factor (BAFF) and B-cell maturation antigen were increased in multiple myeloma cells as compared with those in normal controls PMID: 26914861
  56. we consider recent observations of BAFF and its receptors in RA progression, as well as the effects of BAFF on the cell-cell interactions network. We also summarize the clinical development of BAFF antagonists for the treatment of RA PMID: 26198030
  57. BAFF gene expression play an important role with transplant tolerance in patients receiving kidney transplants. PMID: 26461968
  58. role of TLR3/BAFF axis in IgA class-switch recombination of IgA nephropathy PMID: 26417991
  59. results indicate that analysis of serum concentrations of BAFF and APRIL potentially represents a useful tool for the assessment of AIHA disease activity and progression. PMID: 26243624
  60. Abnormal levels of BAFF/APRIL in pediatric acute lymphoblastic leukemia suggest that BAFF/APRIL are associated with the development and progression of this disease in children PMID: 26950089
  61. Serum BAFF is unexpectedly found to be decreased in serum in response to the T cell independent immunogen Pneumovax 23. PMID: 27031098
  62. BAFF and BAFF-R are expressed in the thyrocytes derived from patients with either autoimmune thyroid disorders or multinodular goiter, as well in the infiltrating immune cells of Graves' disease and Hashimoto's thyroiditis PMID: 26214745
  63. The SNP BLYS -871C>T was associated with the frequency of IgG responders to PvAMA-1 and PvMSP-119. PMID: 26901523
  64. Serum BAFF was significantly higher in connective tissue disease-interstitial lung disease (CTD-ILD) patients compared to healthy subjects and chronic fibrosing interstitial pneumonia patients. BAFF was strongly expressed in CTD-ILD patient lungs. PMID: 26424433
  65. The present study addresses how blood BAFF levels are associated with the clinical course of relapsing-remitting multiple sclerosis. PMID: 26600308
  66. SNPs rs9514828 (BAFF), rs3803800 (APRIL) and rs4985726 (TACI) may be associated with the risk of development of B-cell chronic lymphocytic leukemia in a Polish population. PMID: 26268376
  67. serum levels elevated in the active phase of MPO-ANCA-associated renal vasculitis PMID: 25567522
  68. BAFF expression is directly induced by type I interferons via IRF1 and IRF2, whereas IRF4 and IRF8 are negative regulators of BAFF expression. PMID: 26590315
  69. Taking all the above together, BAFF is emerging as a biomarker of several malignancies and a possible hallmark of cancer cachexia PMID: 26339644
  70. BAFF expressed by B cells may play a pathogenic role in autoimmune bullous diseases, particularly bullous pemphigoid. PMID: 24758362
  71. Cigarette smoke may hinder early mucosal IgA responses in the lung during influenza virus infection through oxidative inhibition of BAFF, which might contribute to the increased incidence and severity of viral infections in smokers. PMID: 25849069
  72. High BAFF was associated with non-Hodgkin's lymphoma in primary Sjogren's syndrome. PMID: 26359802
  73. BAFF levels are significantly lower in maternal peripheral blood in pre-eclamptic pregnancies. PMID: 25656062
  74. A significant association was found between the BAFF -871C/T promoter polymorphism and MC, which may indicate that BAFF could be a potential therapeutic target in HCV-MC. PMID: 25800158
  75. Data support the hypothesis that B-cell BAFF expression creates a self-perpetuating loop contributing to COPD progression by promoting pulmonary B-cell survival and lymphoid follicle expansion. PMID: 26073875
  76. Negative expression of BAFF-R, but not of BAFF, could be an independent risk factor for progression-free survival and Overall survival in patients with diffuse large B-cell lymphoma treated with standard R-CHOP. PMID: 26327569
  77. BAFF is significantly increased in lungs of patients with COPD and is present around both immune and stromal cells within lymphoid follicles. PMID: 26266827
  78. elevated serum levels in patients with Behcet's disease PMID: 25759827
  79. the elevated presence of APRIL and BLyS in B cell-rich areas of chronically inflamed gingiva suggests that these cytokines may contribute to bone loss by promoting the survival and persistence of RANKL-expressing B cells/plasma cells. PMID: 26150532
  80. Elimination of B lymphocyte stimulator receptor 3 (BR-3) and TACI or BR-3 and BCMA inhibits the development of SLE in NZM mice. PMID: 25989238
  81. Serum BAFF levels are significantly elevated in MM patients when compared to healthy controls, and correlate inversely with overall survival. PMID: 25723853
  82. a critical role of miR-30a-3p in the regulation of BAFF expression PMID: 25360821
  83. BAFF expression is not specific to pseudomonas infection and may be a feature of the cystic fibrosis airway. PMID: 24847941
  84. BAFF levels in chronic HBV infection are elevated, correlated with clinical diseases, and could be used as a biomarker for indicating disease mechanisms, activity, and diagnosis. PMID: 24811691
  85. we confirmed the prognostic significance of soluble BLyS levels with regard to TFT in CLL patients, and, more importantly, we showed for the first time that sTACI is a powerful prognostic marker, related to parameters of disease activity PMID: 25162001
  86. our findings demonstrate that the lymphoid microenvironment promotes survival, proliferation and progression of CLL cells via chronic activation of BCR, BAFF/APRIL and NF-kappaB activation while suppressing the immune response. PMID: 24800836
  87. TLR9 mRNA and protein levels in peripheral blood mononuclear cells as well as serum levels of BAFF were significantly increased and may be associated with the overexpression of serum IgA1 in patients with immunoglobulin A nephropathy. PMID: 24993857
  88. HIV-1-triggered release of interferon-alpha/interferon-beta by plasmacytoid dendritic cells induces BAFF production in monocytes. PMID: 25637018
  89. promotes Th17 cell-mediated immunity in Helicobacter pylori-induced gastritis PMID: 25339679
  90. Activation of human cytomegalovirus enhance the activation of BAFF expression in renal transplant recipients. PMID: 24931438
  91. Higher serum concentrations of BAFF in acute myeloid leukemia patients predicted a shorter overall survival. PMID: 25085377
  92. Irrespective of HLA Ab status, sBAFF levels progressively increased in all patients in the first posttransplant year, thereafter reaching a plateau. PMID: 25033258
  93. that BAFF can promote T cell activation, proliferation and differentiation. PMID: 24411564
  94. Availability of BAFF determines BAFF-R and TACI expression on B cells in common variable immunodeficiency. PMID: 24809296
  95. B lymphocyte stimulator (BLyS) is increased in human obesity. PMID: 24728308
  96. these findings suggest the implication of the host's genetic background in pSS-related lymphomagenesis. The interaction of pSS-related BAFF gene haplotypes together with distinct BAFF genetic variants appears to contribute to this complication. PMID: 23845207
  97. Our study demonstrates that BR3 is involved in the survival of cultured epithelial cells due to an autocrine effect of BAFF. PMID: 24602383
  98. Letter: BAFF splice variants area differential expressed, but in a comparable ratio, in healthy controls and myositis patients. PMID: 25152030
  99. TNFRSF13C expression is reduced on CD19(+) cells in active ITP patients. This down-regulation occurs through a post-transcriptional mechanism and could be a consequence of chronic increase of TNFSF13B. PMID: 24889407
  100. In patients who did not develop chronic graft-versus-host disease, there is a temporal pattern of gradually decreasing BAFF levels as B cell numbers increase after myeloablative conditioning. PMID: 24462743

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Subcellular Location Cell membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Tumor necrosis factor ligand superfamily member 13b, soluble form: Secreted
Protein Families Tumor necrosis factor family
Tissue Specificity Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver,
Database Links

HGNC: 11929

OMIM: 603969

KEGG: hsa:10673

STRING: 9606.ENSP00000365048

UniGene: Hs.525157

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