Human Bleomycin hydrolase(BLMH) ELISA kit

Code CSB-EL002716HU
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human BLMH ELISA Kit was designed for the quantitative measurement of Human BLMH protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 28 pg/mL-1800 pg/mL and the sensitivity is 7 pg/mL.

Target Name bleomycin hydrolase
Alternative Names BH ELISA Kit; Bleomycin hydrolase ELISA Kit; BLM hydrolase ELISA Kit; BLMH ELISA Kit; BLMH_HUMAN ELISA Kit; BMH ELISA Kit; EC 3.4.22.40 ELISA Kit
Abbreviation BLMH
Uniprot No. Q13867
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 28 pg/mL-1800 pg/mL
Sensitivity 7 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human BLMH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %110
Range %107-113
1:2Average %89
Range %84-93
1:4Average %85
Range %81-89
1:8Average %86
Range %83-89
Recovery
The recovery of human BLMH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9491-99
EDTA plasma (n=4)105101-109
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
18002.364 2.329 2.347 2.225
9001.526 1.568 1.547 1.425
4500.961 0.975 0.968 0.846
2250.592 0.578 0.585 0.463
112.50.383 0.372 0.378 0.256
560.298 0.271 0.285 0.163
280.197 0.189 0.193 0.071
00.121 0.123 0.122
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.
Gene References into Functions
  1. the BLMH gene single nucleotide polymorphism A1450G (rs1050565) influences BLMH activity and late pulmonary toxicity. PMID: 27327270
  2. Ubc9 plays different roles of action in antitumor agents in chemotherapy. The process requires bleomycin hydrolase and poly(ADP-ribose) polymerase-1. The results are beneficial to deeply understanding of Ubc9 functions and for precise prediction of chemotherapy outcomes in tumors. PMID: 27878232
  3. Bleomycin hydrolase downregulation in lesional skin of adult atopic dermatitis patients is independent of filaggrin gene mutations PMID: 25240784
  4. This study findings suggest that Blmh interacts with diverse cellular processes from energy metabolism and anti-oxidative defenses to cell cycle, cytoskeleton dynamics, and synaptic plasticity essential for normal brain homeostasis. PMID: 24496069
  5. We also detected significant association between XRCC1, XRCC3, and BLHX polymorphisms and a high frequency of chromosomal damage PMID: 24615029
  6. The caspase-dependent cleavage of BLH was confirmed by cleavage of partly-purified human bleomycin hydrolase with caspase-3. PMID: 23708668
  7. BH may play an important role during the late stage of epidermal differentiation. PMID: 22037625
  8. present study suggests that our new method can detect novel genes of interest and that BLMH is a suppressor gene in HCC PMID: 21943823
  9. This first report on BLMH carrier status in Tunisia shows o association between carrying the BLMH-G genotype and Alzheimer's disease in epsilon4 negative or positive subjects. PMID: 20198498
  10. Cysteine proteases bleomycin hydrolase and cathepsin Z mediate N-terminal proteolysis and toxicity of mutant huntingtin. PMID: 21310951
  11. BH activity and expression were markedly decreased in AD lesional skin, suggesting a defect of the filaggrin degradation pathway in AD. PMID: 21190945
  12. Polymorphism is associated with neurodegenerative diseases, notably Alzheimer disease. PMID: 12604387
  13. Significant effect of BLHX variant alleles (A/G, G/G) on the chromosome damage induced by bleomycin. PMID: 18082847
  14. The homozygous variant G/G of BLMH gene SNP A1450G is associated with reduced survival and higher prevalence of early relapses in TC patients treated with bleomycin-containing chemotherapy. PMID: 18398146

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Subcellular Location Cytoplasm. Cytoplasmic granule.
Protein Families Peptidase C1 family
Database Links

HGNC: 1059

OMIM: 602403

KEGG: hsa:642

STRING: 9606.ENSP00000261714

UniGene: Hs.371914

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