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| Code | CSB-E08122h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human BMP-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 98-107 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 87-98 | |||||
| The recovery of human BMP-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-100 | ||||
| EDTA plasma (n=4) | 88 | 82-92 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The human bone morphogenetic protein 7 (BMP7) ELISA Kit is used to quantitatively measure human BMP7 concentrations in serum, plasma, or cell culture supernates. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of BMP7 in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.
BMP7 is a potent anti-inflammatory growth factor that plays an important role in various biological processes, including embryogenesis, hematopoiesis, neurogenesis, and skeletal morphogenesis. BMP7 stimulates the target cells by binding to specific membrane-bound receptor BMPR2 and transduces signals through Smads and MAPK pathways. It maintains multiple physiological processes such as bone development, fracture healing, and differentiation of brown adipose tissue in the body. Downregulation of BMP7 is linked to various diseases including osteoporosis, CVD, and diabetes.
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