Product Details

Purity

≥ 95% as determined by SDS-PAGE.

Endotoxin

≤10 EU/mg by the LAL method

Activity

Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.02-0.04 μg/ml.

Target Names

BMP-7

Uniprot No.

P18075

Alternative Names

Bone morphogenetic protein 7; BMP-7; Osteogenic protein 1 (OP-1); Eptotermin alfa; BMP7; OP1

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

M+316-431aa

Target Protein Sequence

MANVAENSSSDQRQACKKHELYVSFRDLGWQDWIIAPEGYAAYYCEGECAFPLNSYMNATNHAIVQTLVHFINPETVPKPCCAPTQLNAISVLYFDDSSNVILKKYRNMVVRACGCH

Mol. Weight

13.1 kDa

Protein Length

Partial

Tag Info

Tag free

Form

Lyophilized
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered solution containing 0.085% TFA, 30%ACN,5% mannitol

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

BMP7 plays a central role in osteogenic and nephrogenic lineage commitment, making precise control of its bioactivity essential for differentiation studies and regenerative medicine models. This mammalian-expressed protein, spanning residues 316–431 of the mature signaling domain, induces alkaline phosphatase production in ATDC-5 chondrogenic cells with an ED50 of 0.02–0.04 μg/ml, providing quantitative confirmation of receptor-binding potency suitable for osteogenic differentiation assays and stem cell lineage specification experiments. The choice of mammalian expression ensures native post-translational modifications that preserve the dimeric structure and receptor engagement kinetics required for accurate dose-response profiling in proliferation assays, receptor-ligand competition studies, and in vivo bone regeneration models. Purity exceeding 95% by SDS-PAGE and endotoxin levels ≤10 EU/mg align with standards expected in primary cell culture work, supporting use in mechanistic studies of TGF-β superfamily signaling and antibody validation platforms where contaminant interference must be minimized.

Target Background

Function

Growth factor of the TGF-beta superfamily that plays important role in various biological processes, including embryogenesis, hematopoiesis, neurogenesis and skeletal morphogenesis. Initiates the canonical BMP signaling cascade by associating with type I receptor ACVR1 and type II receptor ACVR2A. Once all three components are bound together in a complex at the cell surface, ACVR2A phosphorylates and activates ACVR1. In turn, ACVR1 propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. For specific functions such as growth cone collapse in developing spinal neurons and chemotaxis of monocytes, uses also BMPR2 as type II receptor. Can also signal through non-canonical pathways such as P38 MAP kinase signaling cascade that promotes brown adipocyte differentiation through activation of target genes, including members of the SOX family of transcription factors.

Gene References into Functions

Study results indicated that BMP7 serves a key function in regulating the progression of small-cell lung cancer. PMID: 30015928
in this study, the influence of IGF-1 and BMP-7 in different concentrations on the osteogenic differentiation of two human MSC-subtypes, isolated from reaming debris (RMSC) and iliac crest bone marrow (BMSC) has been assessed PMID: 29874864
BMP7 contributes to epithelial-to-mesenchymal transition-like responses and plays a role equivalent to TGF-β in the course of corneal wound healing. PMID: 29747422
On the contrary, BMP7 specific antibody inhibits the HNK-induced activation of p53 in colon cancer cells and partly decreases the total level of p53. Our findings suggested that HNK may be a promising anticancer drug for CRC; activation of p53 plays an important role in the anticancer activity of HNK, which may be initialized partly by the HNK-induced upregulation of BMP7. PMID: 28731124
All isoforms of type I and type II BMP receptors were expressed in both Ca9-22 and HSC3 cells and BMP7 stimulation resulted in the phosphorylation of Smad1/5/8 in both cell lines PMID: 28812969
the results of the present study indicate that BMP7 may inhibit excessive scar formation via activation of the BMP7/Smad1/5/8 signaling pathway. PMID: 28627680
BMP7, in particular combined with MSC, seems to have a favourable application also in periodontal regeneration. PMID: 28735626
Implantation of morphogenetic protein-7 (BMP-7) gene activated fat tissue fragments can elicit regeneration of large bone defects in rats and could become a clinically expeditious strategy for in vivo bone tissue engineering. PMID: 27373764
the present results showed that OP-1 might serve as a biochemical parameter for determining disease severity in primary knee Osteoarthritis (OA). Further studies with larger sample size need to be carried out to confirm OP-1 as a marker of disease status. Studies are required to be done to examine the genetic and lifestyle factors that may contribute to the development of knee OA PMID: 28474632
BMP7-Based Functionalized Self-Assembling Peptides Protect Nucleus Pulposus-Derived Stem Cells From Apoptosis In Vitro PMID: 27582519
results showed that the expression of cartilage-associated markers in ESC-MSCs induced by the TGFbeta1 and BMP7 combination was increased compared to induction with TGFbeta1 alone. The TGFbeta1 and BMP7 combination upregulated the expression of TGFbeta receptor and the production of endogenous TGFbetas compared to TGFbeta1 induction. PMID: 27292615
Overexpression of truncated ALK5 in a B-cell line counteracted BMP-7-induced apoptosis, whereas overexpression of truncated ALK4 had no effect. PMID: 28489883
study suggests that the common genetic polymorphisms of BMP7 gene are not major contributors to variations in Bone Mineral Density or osteoporotic fracture in postmenopausal Chinese women. PMID: 27264311
Patients with hereditary pulmonary arterial hypertension had significantly higher BMP7 concentrations than patients with idiopathic pulmonary arterial hypertension and control subjects. PMID: 27001265
Expression of bone morphogenetic protein 7 ligand was significantly increased in patients with abnormal uterine bleeding. Study demonstrates that bone morphogenetic protein 7 is a promising target for future investigation and pharmacologic treatment of abnormal uterine bleeding. PMID: 28142396
BMP-7 directly upregulates adhesion and migration of human monocytic cells via activation of beta 2 integrins, Akt, and FAK. PMID: 27669124
results showed that stimulation by BMP-7 leads to an increased osteogenic potential of Mesenchymal stem cells. PMID: 28009419
The disequilibrium between BMP-7 and TGF-beta signals plays a relevant role in the LV remodelling response to haemodynamic stress in aortic stenosis patients with left ventricular hypertrophy/dysfunction. PMID: 27068510
BMP7 may partly mediate the antiproliferative effect of oridonin by activating p38 MAPK in colon cancer cells. PMID: 26986967
Data show that interaction of fibrillin-1 with the bone morphogenetic protein 7 (BMP-7) complex results in a conformational change. PMID: 27059954
the role of BMP-7 and its downstream signals in the neuroprotective effects of oxygen-glucose deprivation preconditioning PMID: 26385023
miR-137/BMP7 axis could contribute to the progression of non-small cell lung cancer. PMID: 26617798
Inhibition of BMP7 expression mediated by KDM5C promotes neoplasm invasion in hepatocellular carcinoma cells. PMID: 26503415
Adenovirus-mediated FoxC2 expression enhances BMP7-facilitated anabolism in nucleus pulposus cells of the intervertebral discs. PMID: 26824865
ANGPTL4 might promote metastasis and might inhibit apoptosis of colorectal cancer cells by up-regulation of BMP7. PMID: 26417691
MiR-22 promotes the development of liver cirrhosis through BMP7 suppression. PMID: 26112332
Transfection of HOXA13 in HKCs could inhibit the degree of EMT induced by albumin-overload, possibly by increasing BMP-7 expression. PMID: 26695677
rs6127973 may be a risk factor of being Nonsyndromic orofacial clefts and confirmed the role of BMP7 gene in orofacial deformity from Western Han Chinese PMID: 25662552
OP-1 levels detected by a polyclonal antibody were found to be much higher than the reference range and correlated positively with the severity of osteoarthritis PMID: 25091889
Exposure of adipogenic stem cells to BMP7 promoted gene expression signatures of brown adipocytes. PMID: 25534037
BMP-7 improves proliferation and hematopoietic reconstitution potential of ex vivo expanded cord blood-derived CD34(+). cells.( PMID: 25192984
hyaluronan regulates bone morphogenetic protein-7-dependent prevention and reversal of myofibroblast phenotype PMID: 25716319
Bone morphogenetic protein 7 increased vascular endothelial growth factor-a expression in human granulosa cells and VEGF receptor expression in endothelial cells to stimulate angiogenesis. PMID: 24023033
Study highlights the potential importance of RGMb in propagating pro-angiogenic effects of HGF and BMP-7. PMID: 24970050
BMP-7 may play anti-inflammatory and anti-fibrogenic roles in the pathogenesis of chronic hepatitis B infection. PMID: 24820464
BMP-7 inhibits TGF-beta-induced cholangiocarcinoma cell migration through inhibition of TGF-beta-mediated Twist and N-cadherin expression PMID: 24969562
BMP-7 represses albumin-induced chemokine synthesis in kidney tubular epithelial cells through destabilization of NF-kappaB-inducing kinase. PMID: 24418819
BMP7 expression correlates with secondary drug resistance in mantle cell lymphoma. PMID: 24069261
Expression and purification of recombinant human bone morphogenetic protein-7 in Escherichia coli. PMID: 24117149
These results suggested an association between a BMP7 single nucleotide polymorphism and selective serotonin reuptake inhibitors treatment response. PMID: 23697978
These findings indicate that BMP-7 mediates ischemic preconditioning -induced tolerance to cerebral ischemia/reperfusion, probably through inhibition of apoptosis. PMID: 24184479
BMP7 induces Langerhans cell differentiation and proliferation by activating the BMP type-I receptor ALK3 in the absence of canonical TGF-beta1-ALK5 signaling. PMID: 24190429
summarize recent knowledge of BMP-7 function and discuss attempts to use this cytokine as a drug to reverse TGF-beta-induced fibrogenesis PMID: 23747893
Exogenous BMP-7 significantly decreased the degree of hepatic fibrosis in both the acute and chronic stages of hepato-schistosomiasis, and the regulatory mechanism may involve the TGF-beta/Smad signaling pathway. PMID: 23539268
The anti-fibrogenic mechanism of rhBMP-7 in the rat liver fibrosis was depended on the reduction of TGF-b1 overexpression and the inhibition of TGF-b1 triggered intracellular signalling in hepatic cells. PMID: 23471555
Nucleus pulposus cells expressing hBMP7 can prevent the degeneration of allogenic intervertebral disc degeneration in a canine transplantation model. PMID: 23580474
BMP7 modulates the expression of E-cadherin and MMP-9, and by which mechanism it may regulate cell migration and metastasis of esophageal squamous cell carcinoma. PMID: 23504348
A novel human bone morphogenetic protein-7 variant with an enriched heparin-binding site PMID: 23888776
There is efficient and reproducible induction of SZP/lubricin accumulation by TGF-beta1 and BMP-7 in skeletal muscle-derived mesenchymal stem cells. PMID: 22490392
monoubiquitinated SMAD6 impairs the binding affinity of non-modified SMAD6 to the BMP type I receptor. Moreover, UBE2O and SMAD6 cooperated in the regulation of BMP7-induced adipogenesis PMID: 23455153

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Subcellular Location

Secreted.

Protein Families

TGF-beta family

Tissue Specificity

Expressed in the kidney and bladder. Lower levels seen in the brain.

Database Links

HGNC: 1074

OMIM: 112267

KEGG: hsa:655

STRING: 9606.ENSP00000379204

UniGene: Hs.473163

Code	CSB-MP002744HU-WD
Abbreviation	Recombinant Human BMP-7 protein, partial (Active)
MSDS	MSDS Datasheet
Size	Inquiry
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Bone morphogenetic protein 7 (BMP7)，Partial (Active)

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