| Code | CSB-E04501h |
| Size | 96T,5×96T,10×96T |
| Trial Size |  |
| Have Questions? | Leave a Message or Start an on-line Chat |
| Description |
The Human BDNF ELISA Kit allows for the in vitro quantitative determination of BDNF concentrations in serum, plasma, cell culture supernates, or tissue homogenates. This kit exclusively recognizes human BDNF. BNDF is ubiquitously expressed in the developing and adult mammalian brain and plays important roles in neuronal development, differentiation, neural regeneration, synaptic transmission, maintenance, plasticity, neurogenesis, and control of short- and long-lasting synaptic interactions involving memory and cognition. BDNF signals through its receptor NTRK2. Low BDNF or NTRK2 expression has been identified in multiple brain disorders and during normal brain aging, which is usually accompanied by mild brain atrophy, impaired neuronal function, and synaptic loss. Studies have shown that BDNF potentiates hippocampal long-term potentiation (LTP), elevates the release of glutamate, and has critical roles in the formation and recall of spatial memory. The detection mechanism of this kit is based on the Sandwich-ELISA technique. BDNF in the sample is bound to the capture antibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled BDNF antibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with BDNF content in the sample. The kit has been quality-controlled with high sensitivity, strong specificity, good linearity, high precision, high recovery, and high lot-to-lot consistency. |
| Target Name | brain-derived neurotrophic factor |
| Alternative Names | Abrineurin ELISA Kit; ANON2 ELISA Kit; BDNF ELISA Kit; BDNF_HUMAN ELISA Kit; Brain Derived Neurotrophic Factor ELISA Kit; Brain-derived neurotrophic factor ELISA Kit; BULN2 ELISA Kit; MGC34632 ELISA Kit; Neurotrophin ELISA Kit |
| Abbreviation | BDNF |
| Uniprot No. | P23560 |
| Species | Homo sapiens (Human) |
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range | 0.3125 ng/mL-20 ng/mL |
| Sensitivity | 0.063 ng/mL |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cardiovascular |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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| Linearity | To assess the linearity of the assay, samples were spiked with high concentrations of human BDNF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  |
| Typical Data | These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed |
| Troubleshooting and FAQs |
ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
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| Function (From Uniprot) |
Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.; Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse.
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| Gene References into Functions |
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| Involvement in disease | Bulimia nervosa 2 (BULN2); Congenital central hypoventilation syndrome (CCHS) |
| Subcellular Location | Secreted.; [BDNF precursor form]: Secreted. |
| Protein Families | NGF-beta family |
| Tissue Specificity | Detected in blood plasma and in saliva (at protein level). Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta. |
| Database Links |
HGNC: 1033 OMIM: 113505 KEGG: hsa:627 STRING: 9606.ENSP00000414303 UniGene: Hs.502182 |
