Human C-type lectin domain family 9 member A(CLEC9A) ELISA kit

Instructions
Code CSB-EL005542HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name C-type lectin domain family 9, member A
Alternative Names C type lectin domain family 9; member A ELISA Kit; C-type lectin domain family 9 member A ELISA Kit; CLC9A_HUMAN ELISA Kit; CLEC9A ELISA Kit; DNGR1 ELISA Kit; HEEE9341 ELISA Kit; PRO34046 ELISA Kit; UNQ9341 ELISA Kit
Abbreviation CLEC9A
Uniprot No. Q6UXN8
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CLEC9A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 89
Range % 85-93
1:2 Average % 104
Range % 100-108
1:4 Average % 89
Range % 85-93
1:8 Average % 108
Range % 104-112
Recovery
The recovery of human CLEC9A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 88 84-93
EDTA plasma (n=4) 106 103-109
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.273 2.109 2.191 2.038
10 1.575 1.604 1.590 1.437
5 1.179 1.205 1.192 1.039
2.5 0.825 0.825 0.825 0.672
1.25 0.525 0.532 0.529 0.376
0.625 0.375 0.368 0.372 0.219
0.312 0.264 0.261 0.263 0.110
0 0.154 0.152 0.153  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.
Gene References into Functions
  1. Clec9a is responsible for the antigen cross-presentation of dendritic cell subsets and may be a target of immunotherapy PMID: 27250027
  2. findings demonstrate that CLEC9A is a specialized receptor that modulates the innate immune response when there is a mycobacterial infection PMID: 29065139
  3. Data indicate that blood dendritic cell antigen 3 BDCA3(+) and C-type lectin domain family 9, member A CLEC9A(+) dendritic cells (DC) are of major importance in the induction of anti-viral and anti-tumor immunity. PMID: 24910448
  4. Activated dendritic cell subsets expressing CD141/CLEA9A/CD1c, likely recruited into the tubulointerstitium, are positioned to play a role in the development of fibrosis and, thus, progression to chronic kidney disease. PMID: 24049150
  5. study showsn that the DNGR-1 ligand is preserved from yeast to man and corresponds to the F-actin component of the cellular cytoskeleton; identification of F-actin as a DNGR-1 ligand suggests that cytoskeletal exposure is a universal sign of cell damage that can be targeted by the innate immune system to initiate immunity PMID: 22483800
  6. High expression of DNGR-1 specifically and universally identifies a unique dendritic cells subset in mouse and humans. PMID: 22442345
  7. propose that Clec9A provides targeted recruitment of the adaptive immune system during infection and can also be utilized to enhance immune responses generated by vaccines. PMID: 22483802
  8. Data show that CLEC9A is only expressed on immature BDCA3(+) myeloid dendritic cells (mDCs). PMID: 22234694
  9. For production of cytotoxic T cells, transgenic DEC-205 and Clec9A, but not Clec12A, are effective targets for antibody-mediated delivery of antigens for vaccination, although only in the presence of adjuvants. PMID: 21677141
  10. A population of human dendritic cells (DC) that expresses DNGR-1 (CLEC9A) and high levels of BDCA3 and resembles mouse CD8alpha+ DCs in phenotype and function, is characterized. PMID: 20479117
  11. DC, NK lectin group receptor-1 (DNGR-1) is a C-type lectin of the NK cell receptor group PMID: 18497879
  12. CLEC9A functions as an activation receptor PMID: 18408006
  13. analysis of differences between mouse and human Clec9A PMID: 18669894
  14. CLEC9A functions as a SYK-coupled C-type lectin receptor to mediate sensing of necrosis by the principal dendritic-cell subset involved in regulating cross-priming to cell-associated antigens PMID: 19219027
  15. The paper cited (J Clin Invest. 2008 Jun;118(6):2098-110) is the first characterization of human and mouse CLEC9A (a.k.a DNGR-1) PMID: 18497879

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Subcellular Location Membrane, Single-pass type II membrane protein
Tissue Specificity In peripheral blood highly restricted on the surface of BDCA31(+) dendritic cells and on a small subset of CD14(+) and CD16(-) monocytes.
Database Links

HGNC: 26705

OMIM: 612252

KEGG: hsa:283420

STRING: 9606.ENSP00000348074

UniGene: Hs.531189

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