CLEC9A Antibody

Datasheet

Code	CSB-PA740922HA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: MCF7 whole cell lysate, JK whole cell lysate, Raji whole cell lysate, THP-1 whole cell lysate, K562 whole cell lysate,U937 whole cell lysate,Mouse Kidney tissue lysate All lanes: CLEC9A antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 27 kDa Observed band size: 35 kDa Immunofluorescence staining of Hela cells with CSB-PA740922HA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of MCF-7 cells with CSB-PA740922HA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CLEC9A Polyclonal antibody

Uniprot No.

Q6UXN8

Target Names

CLEC9A

Alternative Names

C type lectin domain family 9; member A antibody; C-type lectin domain family 9 member A antibody; CLC9A_HUMAN antibody; CLEC9A antibody; DNGR1 antibody; HEEE9341 antibody; PRO34046 antibody; UNQ9341 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human C-type lectin domain family 9 member A protein (57-241aa)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

CUSABIO's product CSB-PA740922HA01HU is a polyclonal antibody against the CLEC9A protein. It was raised in the rabbit immunized with the recombinant human CLEC9A protein (57-241aa) and purified from rabbit serum by antigen affinity chromatography using the immunizing peptide. This CLEC9A antibody exists as the unconjugated IgG isoform. It was validated for use in ELISA, WB, and IF applications. It is reactive to human- and mouse-derived CLEC9A protein.

CLEC9A protein, also known as DNGR1, plays a critical role in recognizing dead cells and facilitating the processing and cross-presentation of dead cell-associated antigens in a Syk-dependent manner. The CLEC9A protein is also a key defining marker of the conventional DC1 (cDC1) subset. CLEC9A is a group V C-type lectin-like receptor that also functions as an activation receptor and can trigger proinflammatory cytokine generation.

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Target Background

Function

Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.

Gene References into Functions

Clec9a is responsible for the antigen cross-presentation of dendritic cell subsets and may be a target of immunotherapy PMID: 27250027
findings demonstrate that CLEC9A is a specialized receptor that modulates the innate immune response when there is a mycobacterial infection PMID: 29065139
Data indicate that blood dendritic cell antigen 3 BDCA3(+) and C-type lectin domain family 9, member A CLEC9A(+) dendritic cells (DC) are of major importance in the induction of anti-viral and anti-tumor immunity. PMID: 24910448
Activated dendritic cell subsets expressing CD141/CLEA9A/CD1c, likely recruited into the tubulointerstitium, are positioned to play a role in the development of fibrosis and, thus, progression to chronic kidney disease. PMID: 24049150
study showsn that the DNGR-1 ligand is preserved from yeast to man and corresponds to the F-actin component of the cellular cytoskeleton; identification of F-actin as a DNGR-1 ligand suggests that cytoskeletal exposure is a universal sign of cell damage that can be targeted by the innate immune system to initiate immunity PMID: 22483800
High expression of DNGR-1 specifically and universally identifies a unique dendritic cells subset in mouse and humans. PMID: 22442345
propose that Clec9A provides targeted recruitment of the adaptive immune system during infection and can also be utilized to enhance immune responses generated by vaccines. PMID: 22483802
Data show that CLEC9A is only expressed on immature BDCA3(+) myeloid dendritic cells (mDCs). PMID: 22234694
For production of cytotoxic T cells, transgenic DEC-205 and Clec9A, but not Clec12A, are effective targets for antibody-mediated delivery of antigens for vaccination, although only in the presence of adjuvants. PMID: 21677141
A population of human dendritic cells (DC) that expresses DNGR-1 (CLEC9A) and high levels of BDCA3 and resembles mouse CD8alpha+ DCs in phenotype and function, is characterized. PMID: 20479117
DC, NK lectin group receptor-1 (DNGR-1) is a C-type lectin of the NK cell receptor group PMID: 18497879
CLEC9A functions as an activation receptor PMID: 18408006
analysis of differences between mouse and human Clec9A PMID: 18669894
CLEC9A functions as a SYK-coupled C-type lectin receptor to mediate sensing of necrosis by the principal dendritic-cell subset involved in regulating cross-priming to cell-associated antigens PMID: 19219027
The paper cited (J Clin Invest. 2008 Jun;118(6):2098-110) is the first characterization of human and mouse CLEC9A (a.k.a DNGR-1) PMID: 18497879

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Subcellular Location

Membrane; Single-pass type II membrane protein.

Tissue Specificity

In peripheral blood highly restricted on the surface of BDCA31(+) dendritic cells and on a small subset of CD14(+) and CD16(-) monocytes.

Database Links

HGNC: 26705

OMIM: 612252

KEGG: hsa:283420

STRING: 9606.ENSP00000348074

UniGene: Hs.531189

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