Human C4b-binding protein beta chain(C4BPB) ELISA kit

Instructions
Code CSB-EL003954HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name complement component 4 binding protein, beta
Alternative Names C4b binding protein beta chain ELISA Kit; C4b-binding protein beta chain ELISA Kit; C4BP ELISA Kit; C4BPB ELISA Kit; C4BPB_HUMAN ELISA Kit; Complement component 4 binding protein beta ELISA Kit; Complement component 4 binding protein beta chain ELISA Kit; Complement component 4-binding protein beta ELISA Kit; OTTHUMP00000034288 ELISA Kit; OTTHUMP00000034289 ELISA Kit; OTTHUMP00000034375 ELISA Kit; OTTHUMP0000003474 ELISA Kit; Proline rich protein ELISA Kit; PRP ELISA Kit
Abbreviation C4BPB
Uniprot No. P20851
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human C4BPB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1000 Average % 96  
Range % 91-108  
1:2000 Average % 92  
Range % 87-99  
1:4000 Average % 94  
Range % 89-98  
1:8000 Average % 97  
Range % 91-112  
Recovery
The recovery of human C4BPB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 90-105  
EDTA plasma (n=4) 95 89-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
200 2.905 2.875 2.890 2.755  
100 2.489 2.473 2.481 2.346  
50 1.734 1.750 1.742 1.607  
25 0.948 0.957 0.953 0.818  
12.5 0.512 0.506 0.509 0.374  
6.25 0.464 0.471 0.468 0.333  
3.125 0.230 0.234 0.232 0.097  
0 0.133 0.136 0.135    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. It also interacts with anticoagulant protein S and with serum amyloid P component. The beta chain binds protein S.
Gene References into Functions
  1. SAA, PROZ, and C4BPB may serve as new potential biomarkers for TB PMID: 28278182
  2. Binding of complement inhibitor C4b-binding protein to a highly virulent Streptococcus pyogenes M1 strain is mediated by protein H and enhances adhesion to and invasion of endothelial cells. PMID: 24064215
  3. crystallographic data on the structure of CD11c/CD18 and prediction of the secondary structure of the C4BP oligomerization domain, we show that epitopes bound by KIM185 in these proteins are unlikely to share any major structural similarity. PMID: 21856004
  4. C4BP alpha7beta0 subunit [C4BP alpha7beta0 isoform lacking the beta-chain] induces a semimature, tolerogenic state on human monocyte-derived dendritic cells activated by a proinflammatory stimulus. PMID: 23390292
  5. Since FI requires cofactors for its activity we also investigated the binding of purified cofactors C4BP and FH and found acquisition of both proteins, which retained their activity in FI mediated degradation of C3b and C4b. PMID: 22514678
  6. Results demonstrate that successful secretion of complement regulatory protein C4b-binding protein beta-chains depends on intracellular complex formation with vitamin K-dependent protein S, but not on the alpha-chains. PMID: 20693287
  7. Localized via protein S to the surface of apoptotic cells, C4BP is able to interact with complement protein C4b, thus retaining its physiological role in regulation of complement on the apoptotic cell surface. PMID: 12193728
  8. C4BP is composed of seven alpha-chains and a unique beta-chain, each chain comprising repeating complement control protein (CCP) modules, the results suggest that the role of CCP2 in protein S binding is to orient and stabilize CCP1 PMID: 12492479
  9. C4b-binding protein binds to necrotic cells and DNA, limiting DNA release and inhibiting complement activation. PMID: 15967823
  10. analysis of structural basis for C4b-binding protein interaction with streptococcal M protein PMID: 16330538
  11. review of multiple functional roles of the complex of protein S and cd4-binding protein beta chain, such as binding to apoptotoic cells, phagocytosis PMID: 17597997
  12. C4BP levels are increased in patients suffering from primary Sjogren's syndrome PMID: 19284503

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Subcellular Location Secreted
Database Links

HGNC: 1328

OMIM: 120831

KEGG: hsa:725

STRING: 9606.ENSP00000243611

UniGene: Hs.99886

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