Human C4b-binding protein beta chain(C4BPB) ELISA kit

Instructions
Code CSB-EL003954HU
Size 96T,5×96T,10×96T

Product Details

Target Name complement component 4 binding protein, beta
Alternative Names C4BP, C4b binding protein, beta chain|complement component 4 binding protein, beta chain|complement component 4-binding protein, beta
Abbreviation C4BPB
Uniprot No. P20851
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human C4BPB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1000 Average % 96  
Range % 91-108  
1:2000 Average % 92  
Range % 87-99  
1:4000 Average % 94  
Range % 89-98  
1:8000 Average % 97  
Range % 91-112  
Recovery
The recovery of human C4BPB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 90-105  
EDTA plasma (n=4) 95 89-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
200 2.905 2.875 2.890 2.755  
100 2.489 2.473 2.481 2.346  
50 1.734 1.750 1.742 1.607  
25 0.948 0.957 0.953 0.818  
12.5 0.512 0.506 0.509 0.374  
6.25 0.464 0.471 0.468 0.333  
3.125 0.230 0.234 0.232 0.097  
0 0.133 0.136 0.135    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. It also interacts with anticoagulant protein S and with serum amyloid P component. The beta chain binds protein S.
Subcellular Location Secreted
Database Links

HGNC: 1328

OMIM: 120831

KEGG: hsa:725

STRING: 9606.ENSP00000243611

UniGene: Hs.99886

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